中文摘要：采后储藏中，生物控制霉菌毒素产毒真菌,拮抗菌能很好地替代农用化学品。本研究评估水稻源菌株，以便鉴定能抑制储藏稻谷中黄曲霉的生物防治剂。据悉，用Microbacterium testaceum和Pseudomonas protegens作为控制储藏稻谷中产毒黄曲霉的生物防治剂的潜力的报道，本研究尚属首次。
外文摘要：Biological control of mycotoxigenic fungi using antagonistic microbes is a promising alternative to agricultural chemicals for postharvest storage. In this study, we evaluated rice-derived bacterial strains to identify biocontrol agents to inhibit Aspergillus flavus in stored rice grains. Consequently, we obtained three potential biocontrol strains (Microbacterium testaceum KU313, Bacillus megaterium KU143 and Pseudomonas protegens AS15) from 26 tested strains that were prescreened from the 460 strains isolated from rice grains. The three selected strains proved to be effective biocontrol agents showing antifungal activity against A. flavus and good colonisation ability on rice grains, along with inhibition of the fungal growth and aflatoxin production. In particular, P. protegens AS15 greatly inhibited the aflatoxins produced by A. flavus on rice grains to 8.68 (percent aflatoxin reduction relative to control = 82.9%) and 18.05 (68.3 %) ng g−1 dry weight of rice grains, compared with the 50.89 and 56.97 ng g−1 dry weight of rice grains of the MgSO4 control at 1 and 2 weeks after inoculation, respectively. In addition, strain AS15 had a significant ability to not only degrade aflatoxin B1 (the most harmful aflatoxin), but also utilise the toxin for bacterial growth in a nutrient-deficient medium. Therefore, the selected bacterial strains could be environmentally sound alternatives for the management of A. flavus and aflatoxin production by reducing the fungal damage to stored rice grains. This would also reduce the human and animal health hazards associated with the consumption of fungus-contaminated rice grains. To our knowledge, this is the first report of the potential of the bacterial species M. testaceum and P. protegens as biocontrol agents for controlling aflatoxigenic A. flavus on stored rice grains.
外文关键词：Aflatoxin；Aspergillus flavus；Bacillus megaterium；biodegradation；biological control；Microbacterium testaceum；Pseudomonas protegens
作者：Mannaa, M；Oh, JY；Kim, KD
作者单位：Korea Univ
期刊名称：ANNALS OF APPLIED BIOLOGY
期刊影响因子：2.103
出版年份：2017
出版刊次：3
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