中文摘要：本研究描述了一种超灵敏的霉菌毒素玉米烯酮（ZEN）电化学免疫分析检测法。与传统方法相比，建立的免疫传感器表现出高度的灵敏性。应用该方法检测谷物和饲料样品中的ZEN，结果表明样品回收率高，检测结果与高效液相色谱检测结果一致性高。因此间接竞争电化学免疫传感器是一种可行的生物分析工具。
外文摘要：The authors describe an electrochemical immunoassay for ultrasensitive detection of the mycotoxin zearalenone (ZEA). A nanocomposite was prepared from carboxy-functionalized multi-walled carbon nanotubes and chitosan (cMWCNTs/Chit). The morphology and electrochemical performance of the materials was characterized by field-emission scanning electron microscopy, atomic force microscopy, differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy. In this assay, ZEA-BSA conjugated covalently to activated cMWCNTs/Chit film, then the indirect competition between ZEA-BSA and free ZEA when immobilization of excess anti-ZEA. The secondary antibody is labeled with the enzyme alkaline phosphatase which can hydrolyze the substrate 1-naphthylphosphate to produce 1-naphthol which gives a stable and strong anodic electrochemical signal at a low working voltage of 0.3 V (vs. Ag/AgCl). The use of the modified GCE results in a strongly enhanced electrochemical current response. Compared with conventional methods, the established immunosensor exhibited a high level of sensitivity. Under optimal conditions, this immunoassay can quantify ZEA in the 10 pg.mL-1 to 1000 ng.mL-1 concentration range with a detection limit of 4.7 pg.mL-1 and the sensitivity is . The method was applied to the determination of ZEA in cereal and feedstuff samples. Results showed satisfactory recovery and good consistency with high-performance liquid chromatography. Therefore, the indirect competitive electrochemical immunosensor provide a viable tool based on bioanalysis.
外文关键词：DPV；Cereal grains；Fodder；Alkaline phosphatase；alpha-Naphthyl phosphate；Electrochemical label
作者：Xu, Wei；Qing, Ying；Chen, Shuai
作者单位：Chongqing Medical University
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：9
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