中文摘要：本研究描述了一种基于CNT的无标记电化学生物传感器在食品中皮克级致癌物质黄曲霉毒素B1（AFB1）检测中的应用。该方法对玉米粉中AFB1的检测极限较低，可用于检测ELISAs无法检测到的浓度。污染玉米粉中浓度非常低以至于不能用已有的来量化的AFB1。该方法提供了一种即时探针，可用于AFB1的检测。
外文摘要：The authors describe a label-free electrochemical biosensor based on CNTs for picogram range detection of the food carcinogen aflatoxin B1 (AFB1). A gold electrode was modified with a cysteine (Cys) self-assembled layer, and carboxyl-functionalized carbon nanotubes were covalently attached to Cys for the subsequent tethering of antibody against AFB1 (anti-AFB1). Topographical images of the biosensor surface were acquired by atomic force microscopy throughout the modification and assay procedure. Upon exposure to samples containing AFB1, its binding to anti-AFB1 will result in a change in electrical conductivity. The use of CNT warrants enhanced electrical properties, and the charge-transfer resistance (Rct) can be related to the concentration of AFB1. Biosensor selectivity was tested by using samples containing ochratoxin A. The electrode displays a limit of detection as low as 0.79 pg.g(-1), and response is linear in the 0.1 to 20 pg.g(-1) concentration range. The assay was applied to the determination of AFB1 in contaminated corn flour at concentrations so low that they cannot be quantified by established ELISAs. In our perception, this method represents a viable point-of-care probe for detection of AFB1.
外文关键词：AflatoxinB1；Nanobiosensor；Immunoreaction；Mycotoxin；ELISA
作者：Costa, Maurilia P.；Frias, Isaac A. M.；Andrade, Cesar A. S.
作者单位：Universidade Federal de Pernambuco
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：9
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