中文摘要：黄曲霉毒素B1（AFB1）是一种污染食品的常见霉菌毒素，本研究借助于AFB1与适配子间的特异性相互作用，构建了一种新型的、基于通用表面增强拉曼光谱（SERS）的适体传感器平台，用于监测AFB1。研究表明，由于来自报道分子双层膜的作为GDADNTs核心的GNTs的强拉曼增感效应、适配子和超顺磁性CS-Fe3O4的特异性，本研究构建的传感器在AFB1痕量检测方面敏感性好、专一性强；同时适用于其它具有特殊配体的痕量分子的检测。
外文摘要：A novel universal Surface-enhanced Raman Spectroscopy (SERS) based aptasensor platform for the trace detection of Aflatoxin B1 (AFB1), a common food contaminating mycotoxin, has been constructed, with the aid of the specific interaction between AFB1 and aptamers. The amino-terminal aptamer conjugated magnetic-bead (CS-Fe3O4) and the gold nanotriangles (GNTs)-DTNB@Ag-DTNB nanotriangles (GDADNTs) were used as the capturer and the reporter of AFB1, respectively. Under the optimized assay condition, the platform shows a distinguished sensitivity with the LOD as low as 0.54 pg/mL and the linear range from 0.001 to 10 ng/mL, a high stability of the SERS substrate activity remained three months at least, a decent reproducibility with RSD of ca. 5%, and a good selectivity to the general coexisted interferences. The distinguished sensitivity and selectivity for trace AFB1 detection has been achieved mainly due to the strong Raman enhancement effect of GNTs as the kernel for GDADNTs from the double-layer of the reporter molecules, the specificity of aptamer and superparamagnetic CS-Fe3O4 respectively. Furthermore, the proposed SERS aptasensor is universal to other trace molecules detection with the specific aptamers.
外文关键词：SERS；GNTs-DTNB@Ag-DTNB；CS-Fe3O4；AFB1；Aptasensor
作者：Yang, Mingxiu；Liu, Guokun；Mehedi, Hassan Md.
作者单位：Jiangsu University
期刊名称：4.712
期刊影响因子：0.0
出版年份：2017
出版刊次：-
点击下载：一种基于DTNB标记GNTs/Ag核壳纳米三角和CS-Fe3O4磁珠的通用SERS适体传感器在黄曲霉毒素B1痕量检测方面的应用