中文摘要：本文评价了转基因甘蓝型油菜对不同芥菜型油菜的基因渗入。本文在温室中利用人工授粉法对转基因甘蓝型油菜Z7B10（花粉供体）和16个芥菜型油菜品种（包括2个野生品种）间的可交配性进行了评价。研究发现，转基因甘蓝型油菜Z7B10与芥菜型油菜品种间的杂交性差异很大。种间杂交F1的种子萌发率在49%-89.3%。从转基因甘蓝型油菜Z7B10到10个芥菜型油菜品种的自然基因流频率估计在0.08%-0.93%。天然F1杂种高度不育。研究结果表明，转基因甘蓝型油菜对不同芥菜型油菜有低水平的基因渗入，这为转基因甘蓝型油菜和芥菜型油菜在中国的安全共存提供了完善的科学依据。

 
外文摘要： Transgene introgression from transgenic rapeseed (Brassica napus) to different varieties of B.juncea was assessed in this study. Crossability between a transgenic rapeseed line Z7B10 (pollen donor) and 80 cultivars of 16 B.juncea varieties (including two wild accessions) was estimated by artificial pollination in a greenhouse. As a result, interspecific crossability between the transgenic Z7B10 line and the 80 B.juncea cultivars varied considerably, with seeds per flower from 0.00-10.67. Seed germination rates of the interspecific F-1 hybrids ranged from 49.0%-89.3%. The estimated frequencies of natural gene flow from the transgenic Z7B10 line to 10 B.juncea cultivars with different uses in the experiment field varied from 0.08% to 0.93%. The natural F-1 hybrids were highly sterile, with seeds per silique ranging from 0.27 to 1.03. In addition, seeds per flower of hybrid descendants varied from 0.02 to 0.22 when F-1 hybrids were self-pollinated, and those ranged from 0.03 to 0.30 when F-1 hybrids were backcrossed with their corresponding B.juncea parents. Results of this study suggest a low level of transgene introgression from transgenic rapeseed to different B.juncea varieties, which provides a sound scientific basis for the safety management of coexisting transgenic B.napus and B.juncea varieties in China.
外文关键词：Brassica juncea varieties, crossability, gene flow, seeds per flower, transgenic Brassica napus
作者：Tang, Tang; Chen, GuiMin; Bu, CuiPing; Liu, FuXia; Liu, Lei; Zhao, XiangXiang
作者单位：Huaiyin Normal Univ
期刊名称：PLANT BREEDING
期刊影响因子：1.335
出版年份：2018
出版刊次：4
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