中文摘要：本研究利用微卫星标记物和荧光标记引物开发了一个事件特异多重PCR系统，该系统可检测六种不同的转基因动物系，包括人乳铁蛋白转基因牛、人溶菌酶转基因牛、人α-乳清蛋白转基因牛、肌肉抑制素敲除猪、植酸酶转基因猪、ω-3脂肪酸去饱和酶基因转基因猪。多重PCR的毛细管电泳结果表明，靶标片段被成功扩增。研究结果表明，该高通量多重PCR检测系统可用于转基因动物的检验与检疫。
外文摘要：This study aimed to establish an event-specific multiplex PCR system using microsatellite markers and fluorescently labeled primers to detect six different genetically modified (GM) animal lines, including human lactoferrin GM cattle, human lysozyme GM cattle, human a-lactalbumin GM cattle, myostatin knockout pigs, phytase GM pigs, and omega-3 fatty acid desaturase gene GM pigs. Four different microsatellite loci for species identification, along with six GM animal-specific fragments, were selected as targets for primer design. The capillary gel electrophoresis results of multiplex PCR showed that the target fragments were amplified successfully. This high-throughput multiplex PCR detection system can be applied for the inspection and quarantine of GM animals.
作者：Liu, Xiaofei; Qiu, Songyin; Li, Xiaolin; Liu, Dandan; Jing, Hongli; Wang, Qin; Lin, Xiangmei; Pan, Dengke; Shi, Ningning
作者单位：Chinese Academy of Inspection and Quarantine
期刊名称：JOURNAL OF AOAC INTERNATIONAL,
期刊影响因子：0.962
出版年份：2018
出版刊次：5
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