中文摘要：本研究开发了一种新型的研究方法，该方法利用免疫亲和柱（IAC）净化结合液相色谱串联质谱（LC-MS/MS）同步检测动物源食品中莱克多巴胺、氯霉素和玉米赤霉醇类（α-玉米赤霉醇、β-玉米赤霉醇、玉米赤霉酮、α-玉米赤霉烯醇、β-玉米赤霉烯醇和玉米赤霉烯酮）。研究表明，该方法可用于动物源食品中莱克多巴胺、氯霉素和玉米赤霉醇残留量的测定。
外文摘要：A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (alpha-zearalanol, beta-zearalanol, zearalanone, alpha-zearalenol, beta-zearalenol, and zearalenone) in animal-originated foods. The sample was first digested by beta-glucuronidase/sulfatase and then extracted with ethyl acetate-diethyl ether (9:1, v/v). The extracted solution was evaporated to dryness and then the residue was dissolved by 2 mL of 50% acetonitrile solution. After filtration, 1 mL filtrate was diluted to 10 mL with PBS. The reconstituted solution was cleaned up with immunoaffinity column and then analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established method was shown to be sensitive efficient and reliable as indicated by the linearity (r (2) >= 0.9994), precision (RSD <= 1.7%), average recovery (72.3-103.2%), and the limit of detection (0.05-0.10 mu g/kg). The method can be used for determination of trace residues of ractopamine, chloramphenicol, and zeranols in animal-originated foods.
外文关键词：HPLC-MS/MS；Immunoaffinity column；Ractopamine；Chloramphenicol；Zeranols；Animal-originated foods
作者：Sun, Xue；Tang, Qiang；Du, Xiaoli等
作者单位：Chongqing Medical University
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.038
出版年份：2017
出版刊次：10
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