中文摘要：由于第一代和第二代转基因抗药性大豆中的CPP4-EPSPS序列发生了变异，现有的PCR方法无法检测到第二代中的CP4-EPSPS基因。本文开发了一种检测CP4-EPSPS基因的新型实时PCR技术。本文在中国成都的17个市场收集了101豆腐产品，并利用实时和常规PCR技术进行了转基因检测研究。本文开发的实时PCR技术特异性和灵敏性高。研究发现，在101个豆腐样品中，68个（67%）含有的CP4-EPSPS基因，或至少含有1个6转化事件，这表明大量的转基因成分存在于成都市场的豆腐产品中。然而，按照中国标签法规，通用的阳性样品没有标明含有转基因生物。由于地方行政部门的放松管制，进口的转基因大豆很可能被非法分散。因此，在当地环境种植大豆可能存在物种多样性的生态风险。
外文摘要： Owing to the variability in theCP4-EPSPS sequence between the 1st and 2nd generation of genetically modified (GM) herb-tolerant soybean, existing PCR methods were not able to detect the CP4-EPSPS gene in the 2nd generation. Here, we developed a novel real-time PCR method for the detection of the CP4-EPSPS gene. In total, 101 tofu products from family workshops or vendors in 17 local farmers' markets in Chengdu, China, were collected and analyzed, for the first time, for the presence of GM organisms (GMOs) using real-time and conventional PCR methods The real-time PCR method established here showed a high specificity and a sensitivity of 5 copies. Among the 101 tofu samples, 68 (67%) tested positive for the CP4-EPSPS gene, or at least 1 of 6 transformation events, suggesting that a large number of GM components present in tofu products in the Chengdu markets. However, none of the GM-positive samples was labeled as containing GMOs, as required by labeling regulations in China. Because of the relaxed control of the local administrative department, the GM soybeans imported for consumption are very possibly being illegally dispersed. Therefore, there may be an ecological risk to the species diversity of cultivated soybeans in local environments.
外文关键词：Tofu, CP4-EPSPS, Transformation events, Real-time PCR, Conventional PCR, Ecological risk, Food composition, Food analysis
作者：Song, Jun; Song, Qiuchi; Wang, Dong; Zhang, Fuli
作者单位：Sichuan Acad Agr Sci
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS
期刊影响因子：2.752
出版年份：2018
出版刊次：4
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