中文摘要：甜瓜是利用生物技术开发作物的重要靶标。本文用农杆菌介导的遗传转化方法对转基因甜瓜T-1代中的抗真菌蛋白几丁质酶基因的稳定性进行了研究；在Murashige和Skoog（MS）培养基中，用150 mg/L添加卡那霉素萌发了转基因甜瓜植株的T-1种子，并用分子分析方法对T-1植物进行了表达分析。作者用RT-PCR和Western blot方法分析了转基因植株的遗传稳定性。研究发现，所有转基因植株均表型正常、完全育性、生长和其它性状均未见异常反应。研究结果表明，抗真菌蛋白几丁质酶基因在转基因甜瓜中存在并在T-1代中表达。
外文摘要：Melon is an important target for crop development by biotechnological tools. We describe the efficient and reproducible production of stable integration and expression transgenic melon plants after plant genetic transformation derived from mature seeds. To determine stability of the antifungal protein-chitinase gene in the T-1 generation of transgenic melon plants, transformed by co-cultivation with Agrobacterium mediated genetic transformation; the T-1, seeds of transgenic melon plants were germinated in Murashige and Skoog (MS) medium with kanamycin added at 150 mg/L. T-1 plants were tested by molecular analysis including expression analysis. Genetic stability of the transgenic plants was assessed by RT-PCR and western blot analysis. The results showed that all transgenic plants were phenotypically normal, fully fertile and no irregular responses in growth and other traits were observed. This suggests that both the antifungal protein-chitinase genes are present and expressed in the T-1 generation.
 
外文关键词：Genetic transformation, Chitinase, Transgenic melon plants, Transgene stability and expression
作者：Bezirganoglu, Ismail
作者单位：Erzurum Tech Univ
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
期刊影响因子：0.0
出版年份：2018
出版刊次：5
点击下载：转基因甜瓜T-1代抗真菌蛋白几丁质酶基因的稳定性研究