中文摘要：本研究开发了一种快速灵敏的复合成像表面等离子体共振（iSPR）生物传感器测定法，并通过脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）和T-2毒素三种伏马菌素的测定验证其检测限和灵敏度。该方法灵敏度高，符合欧盟委员会（EC）的要求，能够用于监测小麦中的霉菌毒素污染。研究中使用空白小麦和麦穗验证了三种镰刀菌毒素的截留水平，均在EC规定水平（ZEA和T-2毒素100μg/kg）或者EC水平（DON400μg/kg）的1/3。后续将进一步验证该方法用于监测自然污染的小麦样品的可行性。这是金纳米颗粒增强iSPR方法在检测和区分小麦中三种重要镰刀菌毒素方面应用的首次报道。
外文摘要：A rapid, sensitive and multiplexed imaging surface plasmon resonance (iSPR) biosensor assay was developed and validated for three Fusarium toxins, deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin. The iSPR assay was based on a competitive inhibition format with secondary antibodies (Ab(2)) conjugated to gold nanoparticles (AuNPs) used as a signal amplification tag. Signal was amplified nearly 25-fold for DON, 90-fold for ZEA and 12-fold for T-2 toxin assay using Ab(2)-AuNPs. Analyses, including steps to regenerate the sensor, took 17.5 min. The antigen coated sensor chip was used for more than 46 cycles without affecting signal intensity ( < 12%). Matrix matched calibration curves were used to determine Fusarium toxins in wheat. The mean recoveries ranged from 87% to 103% with relative standard deviations of repeatability of less than 5%. The limits of detection were 15 mu g/kg for DON, 24 mu g/kg for ZEA and 12 mu g/kg for T-2 toxin. This provided sufficient sensitivity to monitor contamination of these mycotoxins in wheat in accordance with European Commission (EC) limits. Cut off levels for all three Fusarium toxins were validated using blank wheat and wheat spiked either at the EC regulated levels (100 mu g/kg for ZEA and T-2 toxin) or at one third of the EC level (for DON: 400 mu g/kg). The assay was successfully applied and further validated with naturally contaminated wheat samples. This is the first reported AuNP enhanced iSPR assay to detect and classify three agriculturally important Fusarium toxins in wheat.
外文关键词：Mycotoxins；Fusarium toxins；iSPR；Gold nanoparticle；Wheat；Validation
作者：Hossain, Md Zakir；Maragos, Chris M.
作者单位：United States Department of Agriculture (USDA)
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.78
出版年份：2018
出版刊次：-
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