中文摘要：T-2/HT-2毒素(T-2/HT-2)和赭曲霉毒素A（OTA）是污染各种农产品的霉菌毒素。为了保护消费者的健康，欧盟委员会已经给出了食品中T-2/HT-2污染的指示性限值和OTA污染的最高限值，并要求食品经营者和监管部门对这些有害污染物进行例行检查。本研究开发了T-2/HT-2酶联免疫吸附测定法（ELISAs）和OTA酶联免疫吸附测定法（ELISAs）。研究根据欧盟委员会No 519/2014法规中提出的霉菌毒素测定方法验证指南，对两种方法进行了验证。结果证明，T-2/HT-2 ELISA适用于检测T-2/HT-2靶标浓度（STC）分别在12.5 g/kg和7.5 g/kg以上的谷物和婴儿食品；OTA ELISA适用于检测STC分别在2 g/kg、2.5 g/kg、2.5 g/kg和0.4 ng/Ml以上谷物、咖啡、可可和酒中的OTA。研究通过分析水平测试和参考样品进一步确认了两种ELISAs的准确性。综上所述，本研究开发的方法可用于敏感性高、高通量的农产品检测。
外文摘要：T-2 toxin/HT-2 toxin (T-2/HT-2) and ochratoxin A (OTA) are mycotoxins that can contaminate a variety of agricultural commodities. To protect consumers' health, indicative limits for T-2/HT-2 and maximum limits for OTA have been set by the European Commission, requiring food business operators and controlling agencies to conduct routine checks for the presence of these harmful contaminants. Screening methods are increasingly used for monitoring purposes. Due to the demand for new and improved screening tools, two individual detection methods, T-2/HT-2 and OTA enzyme-linked immunosorbent assays (ELISAs), were developed in this study. The T-2/HT-2 ELISA was based on a T-2 monoclonal antibody with an IC50 (50% inhibitory concentration) of 0.28 ng/mL and 125% cross-reactivity with HT-2. As regards the OTA ELISA, a new sensitive monoclonal antibody specific to OTA with an IC50 of 0.13 ng/mL was produced. Both developed ELISA tests were then validated in agricultural commodities in accordance with the new performance criteria guidelines for the validation of screening methods for mycotoxins included in Commission Regulation (EU) No 519/2014. The T-2/HT-2 ELISA was demonstrated to be suitable for the detection of T-2/HT-2 in cereals and baby food at and above the screening target concentration (STC) of 12.5 g/kg and 7.5 g/kg, respectively. The OTA ELISA was shown to be applicable for the detection of OTA in cereals, coffee, cocoa and wine at and above the STC of 2 g/kg, 2.5 g/kg, 2.5 g/kg and 0.4 ng/mL, respectively. The accuracy of both ELISAs was further confirmed by analysing proficiency test and reference samples. The developed methods can be used for sensitive and high-throughput screening for the presence of T-2/HT-2 and OTA in agricultural commodities.
外文关键词：mycotoxins；enzyme-linked immunosorbent assay；immunoassay；screening
作者：Oplatowska-Stachowiak, Michalina；Kleintjens, Tim；Sajic, Nermin等
作者单位：EuroProxima BV
期刊名称：TOXINS
期刊影响因子：3.03
出版年份：2017
出版刊次：12
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