中文摘要：本研究构建了一种新型夹层式电化学发光（ECL）免疫传感器，该传感器利用四方金红石TiO2介观晶体（TRM）的双重作用，能够实现对玉米赤霉烯酮的（ZEA）的灵敏检测。该传感器具有较低的检测限和较宽的线性范围。
外文摘要：Herein, a new sandwich-type electrochemiluminescence (ECL) immunosensor was established on the dual-roles of tetragonal rutile TiO2 mesocrystals (TRM) to realize the sensitive detection of zearalenone (ZEA). On the one hand, the poly (amidoamine) dendrimers (PAAD)-functionalized TRM, combining the advantages of hyper-branched of PAAD and the high nanoporosity, large surface area of TRM, was served as the sensing matrix to immobilize enough capturing antibody, realizing the signal amplification. On the other hand, TRM was applied as an effective probe platform to absorb Ru (bpy)(3)(2+) and simultaneously immobilize the secondary antibody (Ab(2)). Here, based on the superior properties of TRM and the new bridge of 4-Mercaptophenylboronic acid (4-MPBA) for the high affinity of Ab(2) and TRM, a strong and stable ECL emission was realized. Additionally, cysteamine first emerging as a blocker was proved to sensitize the ECL response of Ru (bpy)(3)(2+), which further amplified the ECL signal, avoiding addition of exogenous coreactants into testing solution. Finally, the sandwich-type ECL immunosensor enabled the real samples detection of ZEA with a lower detection limit and wider linear range.
外文关键词：Electrochemiluminescence；Mycotoxins；Immunosensor；Tetragonal rutile TiO2 mesocrystals；Zearalenone
作者：Zheng, Hongli；Yi, Huan；Lin, Wen等
作者单位：Fujian Normal University
期刊名称：ELECTROCHIMICA ACTA
期刊影响因子：4.798
出版年份：2018
出版刊次：-
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