中文摘要：在商业化之前，需对转基因作物进行评估以确定新表达蛋白的过敏原性。一些监管机构需要对常见过敏原作物（包括大豆）的内源性过敏原进行评估，以确定遗传转化是否增加了内源性过敏原浓度，甚至要求使用来自个别致敏对象的血清进行IgE检测。但对非转基因品种或不同环境条件下内源性过敏原表达的变异性鲜为人知。本文在不同地点研究了非商业性转基因大豆、常规近等基因大豆和五个非转基因商业大豆的IgE与内源性过敏蛋白的结合，并利用11名大豆过敏患者的血清或血浆进行一维（1D）和二维（2D）免疫印迹和ELISA分析。免疫印迹和ELISA检测结果显示，转基因大豆与其常规近等基因大豆的IgE结合情况无明显差异。然而，在不同的非转基因商业大豆品系和不同的种植地点中，观察到IgE结合模式有一些明显差异，这突出了内源性过敏原蛋白的固有变异性。研究结果表明，与非转基因大豆相比，了解内源性过敏原水平的潜在变异性是建立转基因大豆授权标准的必要条件。
外文摘要：Prior to commercialization, genetically modified (GM) crops are evaluated to determine the allergenicity of the newly expressed protein. Some regulators require an evaluation of endogenous allergens in commonly allergenic crops including soybean to determine if genetic transformation increased endogenous allergen concentrations, even asking for IgE testing using sera from individual sensitized subjects. Little is known about the variability of the expression of endogenous allergens among non-GM varieties or under different environmental conditions. We tested IgE binding to endogenous allergenic proteins in an experimental non-commercial GM line, a non-GM near-isoline control, and five non-GM commercial soybean lines replicated at three geographically separated locations. One-dimensional (1D) and two-dimensional (2D) immunoblotting and ELISA were performed using serum or plasma from eleven soybean allergic patients. The results of immunoblots and ELISA showed no significant differences in IgE binding between the GM line and its non-GM near-isoline control. However, some distinct differences in IgE binding patterns were observed among the non-GM commercial soybean lines and between different locations, highlighting the inherent variability in endogenous allergenic proteins. Understanding the potential variability in the levels of endogenous allergens is necessary to establish a standard of acceptance for GM soybeans compared to non-GM soybean events and lines.
外文关键词：Endogenous allergenicity, ELISA, Genetically modified (GM) soybean, Inmunoblot, Natural variation, Safety assessment
作者：Lu, Mei; Jin, Yuan; Ballmer-Weber, Barbara; Goodman, Richard E.
作者单位：University of Nebraska Lincoln
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.778
出版年份：2018
出版刊次：2
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