中文摘要：转Bt基因棉花的广泛种植需要优先评估其对非靶标生物的潜在影响。然而，转Bt基因棉花对许多昆虫的影响实际上是未知的。中黑盲蝽是华南地区危害棉花的主要害虫，甲虫是最古老的棉花授粉者之一。本文通过实验评估了表达Cy1Ac/Cyr2Ab的转Bt基因棉花ZMSJ和表达Cy1Ac/EPSPS的转Bt基因棉花ZMKCKC对成年中黑盲蝽和甲虫的影响。研究发现，对中黑盲蝽和甲虫饲喂转Bt基因棉花叶或花粉，7天后没有任何物种的死亡率出现显著增加。在两个物种中都可以检测到微量的Cy1Ac和Cyr2Ab蛋白，但在体外结合实验中没有发现Cyr1Ac和Cyr2Ab结合蛋白。研究结果表明，饲喂这两个转Bt基因棉花的叶或花粉对中黑盲蝽和甲虫没有毒性作用。
外文摘要：The widespread cultivation of transgenic Bt cotton makes assessing the potential effects of this recombinant crop on non-target organisms a priority. However, the effect of Bt cotton on many insects is currently virtually unknown. The plant bug Adelphocoris suturalis is now a major pest of cotton in southern China and the beetle Haptoncus luteolus is one of the most ancient cotton pollinators. We conducted laboratory experiments to evaluate the toxicity of the Bt cotton varieties ZMSJ, which expresses the toxins Cry1Ac and Cry2Ab, and ZMKCKC, which expresses Cry1Ac and EPSPS, on adult A. suturalis and H. luteolus. No significant increase in the mortality of either species was detected after feeding on Bt cotton leaves or pollen for 7 days. Trace amounts of Cry1Ac and Cry2Ab proteins could be detected in both species but in vitro binding experiments found no evidence of Cry1Ac and Cry2Ab binding proteins. These results demonstrate that feeding on the leaves or pollen of these two Bt cotton varieties has no toxic effects on adult A. suturalis or H. luteolus.
外文关键词：Risk assessment, Non-target insects, Cry toxin, Binding assays
作者：Niu, Lin; Tian, Zhenya; Liu, Hui; Zhou, Hao; Ma, Weihua; Lei, Chaoliang; Chen, Lizhen
作者单位：Huazhong Agr Univ
期刊名称：ENVIRONMENTAL POLLUTION
期刊影响因子：5.099
出版年份：2018
出版刊次：5
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