中文摘要：本研究筛选了45个巴斯利卡塔（意大利）广泛种植的本地甜椒(Capsicum annuum)样品中的17种霉菌毒素及潜在的产毒真菌类属。研究发现阳性样品的百分率很高，部分霉菌毒素的含量也很高，证实了甜椒易受霉菌毒素污染，并要求改进生产和干燥的条件。
外文摘要：Forty-five samples of a landrace of sweet pepper (Capsicum annuum) widely cultivated in Basilicata (Italy) were screened for 17 mycotoxins and potential toxigenic fungal species. Two different LC-MS/MS methods were used for the determination of aflatoxins, ochratoxin A (OTA), Fusarium mycotoxins zearalenone (ZEA), fumonisins (FB1 and FB2), nivalenol (NIV), deoxynivalenol (DON), T-2 and HT-2 toxins and Alternaria mycotoxins altenuene (ALT), alternariol (AOH), alternariol monomethyl ether (AME), tentoxin (TTX) and tenuazonic acid (TeA). Frequency of potential toxigenic fungal species occurrence was: 87% Aspergillus Sect. Nigri; 58% Aspergillus Sect. Flavi; 38% Aspergillus Sect. Circumdati; 42% Alternaria spp.; 33% Penicillium spp. and 20% Fusarium spp. Frequency of mycotoxin occurrence and mean of positives were: 51% OTA, 29.5 mu g/kg, 5 samples above the EU limit of 20 mu g/kg; 31% aflatoxins, 12.8 mu g/kg, two samples above the EU limit of 5 mu g/kg for aflatoxin B1; 91% ZEA, 1.4 mu g/kg; 78% FB2, 7.6 mu g/kg; 58% FB1, 22.8 mu g/kg; 38% NIV, 39.5 mu g/kg; 36% DON, 6.9 mu g/kg; 20% T-2 toxin, 5.6 mu g/kg and 22% HT-2 toxin, 13.8 mu g/kg. For the Alternaria mycotoxins, 100% of samples contained TeA, 4817.9 mu g/kg; 93% TTX, 29.7 mu g/kg; 56% AOH, 114.4 mu g/kg; 33% AME, 13.0 mu g/kg and 9% ALT, 61.7 mu g/kg. Co-occurrence of mycotoxins in each sample ranged from 2 to 16 mycotoxins (mean 7). No statistical correlation was found between moulds and their mycotoxins occurrence. Within the four groups of peppers collected herein (fresh, dried, grounded and fried) higher percentages of contamination and mycotoxin levels were measured in grounded peppers, whereas much lower values were observed for fried peppers. The high percentages of positive samples and the high levels of some mycotoxins observed in this study confirm the susceptibility of peppers to mycotoxin contamination and claims for an improvement of the conditions used during production and drying process.
外文关键词：LC-MS/MS；multi-mycotoxin analysis；processing；drying；toxigenic fungi
作者：Gambacorta, L；Magista, D；Perrone, G；Murgolo, S；Logrieco, AF；Solfrizzo, M
作者单位：Natl Res Council Italy CNR
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2018
出版刊次：1
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