中文摘要：基因叠加是农业生物技术的发展趋势。叠加转基因植物的非预期效应是公众和研究者所考虑的安全问题。组学技术为评估意外影响提供了有用的工具。本文通过转基因玉米品种12-5和IE034的杂交，获得了抗虫性和抗草甘膦EPSPS基因叠加的转基因玉米12-5xIE034，并对中国不同省区的转基因玉米品种112-5xIE034、12-5、IE034和常规品种进行了转录组和代谢组学分析。转录组结果发现，九个玉米品种在基因表达上存在明显差异。12-5xIE034与其亲本及转基因受体之间存在3561-3538个差异表达基因，远小于其与常规玉米品种差异表达基因的数量。聚类分析表明，12-5xIE034与其亲本之间存在密切关系。代谢组结果发现，九个玉米品种中不同代谢物的数量在0-240之间。与其亲本、12-5和IE034相比，112-5xIE034分别含有15和112种不同代谢物。聚类分析表明，12-5xIE034与其亲本间的差异小于与其它玉米品种间的差异。本文为评估叠加转基因植物的非预期效应提供了科学数据。
外文摘要：Gene stacking is a developing trend in agricultural biotechnology. Unintended effects in stacked transgenic plants are safety issues considered by the public and researchers. Omics techniques provide useful tools to assess unintended effects. In this paper, stacked transgenic maize 12-5xIE034 that contained insecticidal cry and glyphosate tolerance G10-epsps genes was obtained by crossing of transgenic maize varieties 12-5 and IE034. Transcriptome and metabolome analyses were performed for different maize varieties, including 12-5xIE034, 12-5, IE034, and conventional varieties collected from different provinces in China. The transcriptome results were as follows. The nine maize varieties had obvious differences in gene expression. There were 3561-5538 differentially expressed genes between 12-5xIE034 and its parents and transgenic receptor, which were far fewer than the number of differentially expressed genes in different traditional maize varieties. Cluster analysis indicated that there were close relationships between 12-5xIE034 and its parents. The metabolome results were as follows. For the nine detected maize varieties, the number of different metabolites ranged from 0 to 240. Compared with its parents, 12-5 and IE034, the hybrid variety 12-5xIE034 had 15 and 112 different metabolites, respectively. Hierarchical cluster analysis with Pearson's correlation analysis showed that the differences between 12-5xIE034 and its parents were fewer than those between other maize varieties. Shikimate pathway-related genes and metabolites analysis results showed that the effects of hybrid stacking are less than those from transformation and differing genotypes. Thus, the differences due to breeding stack were fewer than those due to natural variation among maize varieties. This paper provides scientific data for assessing unintended effects in stacked transgenic plants.
外文关键词： breeding stack, transgenic maize plant, transcriptome, metabolome, unintended effect
作者：Wang, Xu-jing; Zhang, Xin; Yang, Jiang-tao; Wang, Zhi-xing
作者单位：Chinese Acad Agr Sci
期刊名称：PLANT JOURNAL
期刊影响因子：5.901
出版年份：2018
出版刊次：5
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