中文摘要：本研究制备并运用氧化石墨烯-金纳米复合材料(GO/AuNCs)为新型荧光猝灭平台，与杂交链式放大(HCR)技术结合，用来超灵敏检测黄曲霉毒素B1(AFB1)。研究表明，在检测霉变农产品中的AFB1时，该方法对通常与AFB1共存的其他干扰剂具有优良的选择性。此外，该方法还能有效地检测花生样品中的AFB1。
外文摘要：Graphene oxide-gold nanocomposites (GO/AuNCs) were prepared and used as a novel fluorescence quenching platform for ultrasensitive detection of aflatoxin B1 (AFB1) coupled with hybridization chain reaction (HCR) amplification. In the designed system, two fluorophore labeled hairpin probes (HP1/HP2) were introduced, and the fluorescence signals of them were effectively quenched due to the adsorption on GO/AuNCs. Associate probe (AP) was used for the specific recognition of AFB1, and the stem-loop structure of it was opened. Meanwhile, the exposed section of AP was utilized as an initiator for the happen of HCR between HP1 and HP2, a strong fluorescence signal was obtained due to the formation of long nicked dsDNA duplex and desorbed of them from the surface of GO/AuNCs. Under the optimal conditions, GO/AuNCs displayed 94% of the quenching efficiency to the fluorescent probes, and a detection limit down to 0.03 pg/mL was obtained for AFB1 detection. In particular, the assay exhibited excellent selectivity for the detection of AFB1 against other interfering agents that normally coexist with AFB1 in mildewed agriculture products. Moreover, the assay could realize the detection of AFB1 effectively in peanut samples.
外文关键词：Graphene oxide-gold nanocomposites；Hairpin probes；Associate probe；Fluorescent sensor；AFB(1) detection；Hybridization chain reaction
作者：Li, ZB；Xue, N；Ma, HY；Cheng, ZY；Miao, XM
作者单位：Jiangsu Normal Univ
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2018
出版刊次：5
点击下载：一种基于氧化石墨烯-金纳米复合材料荧光猝灭的超灵敏开关控制平台，用于检测花生中的黄曲霉毒素B1