中文摘要：最近检测到天然的大麦黑麦麦角污染物震颤帕西林(PAX)，一种吲哚二萜生物碱真菌毒素。为了检查这种毒素转移到啤酒中的可能性，本研究研发了一种灵敏而快捷的免疫化学法（enzyme immunoassay，EIA），来分析工业瓶装啤酒中的PAX。研究表明，该方法适用于筛查啤酒中的PAX。用该方法调查德国市场售卖的38个国内外品牌的瓶装啤酒，其中后者添加了各种调味料，发现所有样品中PAX呈阴性，表明这种毒素不是啤酒中的相关污染物。
外文摘要：The tremorgenic paxilline (PAX), an indole-diterpene alkaloid mycotoxin, was recently detected as a natural contaminant of ergot of barley and rye. To check the possibility of a transfer of this mycotoxin into beer, a rapid and sensitive immunochemical method for the analysis of PAX in commercial bottled beer was developed. A straightforward sample preparation procedure could be established, including degassing, pH adjustment, optional filtering, and finally a 1:5 dilution with a methanolic phosphate-buffered saline solution. Analysis of PAX was performed by a competitive indirect enzyme immunoassay (EIA). The detection limit at a cut-off value of 80% B/B0 of the EIA standard curve was evaluated by analysis of spiked beer. PAX at levels of 3 μg/L and 5 μg/L yielded 69% and 100% positive results, therefore the detection limit in beer was at 5 μg/L. Recoveries of PAX at levels of 5–20 μg/L were 88–97%, coefficients of variation were 17–22%. With these characteristics, the EIA was considered to be a suitable screening method for PAX in beer. A survey of bottled beer from the German market was conducted which included 38 samples of domestic and international brands, the latter containing various flavoring ingredients. All samples were clearly negative for PAX, which indicates that this toxin is not a relevant contaminant in beer.
外文关键词：Paxilline；Tremorgenic mycotoxins；Beer；Food cereals；Enzyme immunoassay
作者：Bauer, JI；Gross, M；Hamscher, G；Usleber, E
作者单位：Justus Liebig Univ Giessen
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：4
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