中文摘要：本研究首次报道用亚临界水萃取-固相萃取-液相色谱-串联质谱检测法检测不同谷物中的6种单端孢霉烯类毒素（脱氧雪腐镰刀菌烯醇、3-葡萄糖苷脱氧雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇、15-乙酰脱氧雪腐镰刀菌烯醇、HT-2毒素和T-2毒素）。研究表明，该方法适用于不同种类的谷物、假种皮和油籽样品，其中玉米、小米和燕麦至少受一种单端孢霉烯类毒素污染。
外文摘要：Subcritical water extraction followed by solid-phase extraction and ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection is reported for the first time for the determination of 6 trichothecenes (deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, HT-2 toxin, and T-2 toxin) from different cereals. Water with 1% formic acid was used as the extraction solvent followed by a solid-phase extraction clean-up, achieving good performance with acceptable extraction recoveries, method detection limits between 0.05 μg kg−1 and 4.0 μg kg−1, and method quantification limits between 0.4 μg kg−1 and 20 μg kg−1. The use of water as the extraction solvent allowed a selective extraction affording low matrix effect levels and the detection and quantification of natural target trichothecenes at very low concentration levels. This extraction method was applied to different cereals, a pseudocereal and an oilseed sample, of which maize, millet, and oat were contaminated by at least one trichothecene.
外文关键词：Trichothecenes；Cereals；Subcritical water extraction；Solid-phase extraction；UHPLC-(ESI)MS/MS
作者：Miro-Abella, E；Herrero, P；Canela, N；Arola, L；Ras, R；Fontanals, N；Borrull, F
作者单位：Univ Rovira & Virgili
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：4
点击下载：用亚临界水萃取-固相萃取-液相色谱-串联质谱法测定谷物基质中的单端孢霉烯类毒素