中文摘要：脱氧雪腐镰刀菌烯醇（DON）是谷物中由镰刀菌产生的主要有毒次生代谢物之一。许多有前途的脱氧雪腐镰刀菌烯醇解毒策略，是利用微生物和酶，将DON转化成无毒的DON代谢物。本研究开发并验证了一种稳健的气相色谱-质谱联用(GC-MS)法，用于同时分析DON及其两种细菌代谢物3-酮基-DON和3-epi-DON。研究表明，该方法能灵敏、精准而稳健地检测液体及其它复杂基质中的DON、3-epi-DON和3-keto-DON，并避免其它化合物的干扰。
外文摘要：Deoxynivalenol (DON) is one of the major toxic secondary metabolites produced by Fusarium fungi in cereal grains. Among the many promising strategies of DON detoxification are the microbial and enzymatic ones, which transform DON to nontoxic DON metabolites. Thus, proper analytical methods are needed for those DON metabolites. In this study, a robust gas chromatography-mass spectrometry (GC-MS) procedure was developed and validated for the simultaneous analysis of DON and two of its bacterial metabolites, 3-keto-DON and 3- epi-DON. The procedure involves a straightforward vacuum drying and derivatization step before the subsequent GC-MS analysis. Following the optimized protocol, DON and these two metabolites were separated on a capillary column within 15 min. The linear ranges for the these compounds were 10 to 2,000 ng mL-1 with correlation coefficients >0.99. For DON, 3- epi-DON, and 3-keto-DON, the limits of detection were 0.8, 3.0, and 0.05 ng mL-1, and the limits of quantification were 2.6, 10.0, and 1.0 ng mL-1, respectively. For all three compounds, the obtained relative standard deviation was 1.2 to 5.5%, and the recovery rates were 89.5 to 103.6%. The developed method was further validated by analyzing DON metabolites resulting from the biotransformation of DON initiated by cell-free lysates of the bacterium Devosia mutans 17-2-E-8. The developed protocol was sensitive, precise, accurate, and robust for the determination of DON, 3- epi-DON, and 3-keto-DON in liquid media and potentially other complex matrices without interference from other compounds.
外文关键词：Deoxynivalenol；3-epi-DON；3-keto-DON；Gas chromatography-mass spectrometry
作者：Tang, KJ；Liu, HZ；Li, XZ；Hassan, YI；Shao, SQ；Zhou, T
作者单位：Jiangxi Agr Univ
期刊名称：JOURNAL OF FOOD PROTECTION
期刊影响因子：1.609
出版年份：2018
出版刊次：2
点击下载：一种同时分析脱氧雪腐镰刀菌烯醇及其细菌代谢物3-酮基-DON和3-epi-DON的高效气相色谱-质谱法