中文摘要：本研究近期在我国河南省，当地以小麦作主食，消费量较高，开展了脱氧雪腐镰刀菌烯醇(DON)残留的风险评估。研发并验证了一种高通量灵敏UPLC-MS/MS法，后接96孔洗脱固相萃取(SPE)，用来测定人体尿液中的DON生物标记。据估计，所有参试者每日可能的平均和中位数摄入量（PDI），达到了1.61μg/kg bw和1.10μg/kg bw，均超过了PMTDI (1μg/kg bw/day)，表明了该地区居民的潜在风险，特别是儿童和青少年。
外文摘要：A risk assessment of deoxynivalenol (DON) was recently conducted for the residents in Henan province, China, where wheat as the staple food are highly consumed. A high-throughput sensitive UPLC-MS/MS method following 96-well μElution solid-phase extraction (SPE) were developed and validated for the determination of DON biomarkers in human urine. Isotope labelled internal standard, 13C-DON, was used for accurate quantification. Urinary samples collected from 151 healthy Chinese aged 2-78 years were processed with and without enzyme hydrolysis to determine total and free biomarkers, respectively. DON, and de-epoxy-deoxynivalenol (DOM-1) to a lesser extent, can be frequently detected in these samples both with and without enzyme hydrolysis. Free DOM-1 was detected at low level in human urine for the first time. Total DON was detected in all samples with a mean concentration at 47.6 ng mL-1. The mean and median probable daily intakes (PDI) for the whole participants, estimated to be 1.61 μg/kg bw and 1.10 μg/kg bw, both exceeded the PMTDI (1 μg/kg bw/day), indicating a potential risk for the residents in this area, especially for children and adolescents.
作者：Deng, CL；Li, CL；Zhou, S；Wang, XD；Xu, HB；Wang, D；Gong, YY；Routledge, MN；Zhao, YF；Wu, YN
作者单位：Minist Hlth
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2018
出版刊次：5
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