中文摘要：由不同类属真菌产生的真菌毒素可共存于谷物及饲料中，对人体和动物都有很高的毒性。为了量化谷物中的多种真菌毒素，本研究开发了一种真菌毒素纸免疫亲和试验。研究表明，该纸免疫亲和试验为检测农产品中多种真菌毒素提供了一种灵敏、快速、准确、稳定而便捷的平台。
外文摘要：Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 μg·kg-1, respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81-86% for deoxynivalenol, 89-117% for zearalenone, 79-86% for T-2 toxin, and 78-83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods.
外文关键词：Paper-based array；Immune-affinity array；Mycotoxins；UV-initiated copolymerization；Chemiluminescent detection
作者：Li, L；Chen, HP；Lv, XL；Wang, M；Jiang, XZ；Jiang, YF；Wang, HY；Zhao, YF；Xia, LR
作者单位：Jiangsu Acad Agr Sci
期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY
期刊影响因子：3.125
出版年份：2018
出版刊次：8
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