中文摘要：2006，转基因番木瓜 ‘华农1号’的栽培成功地控制了华南地区番木瓜环斑病毒引起的破坏性番木瓜环斑病。然而，一些来自广东和海南的转基因番木瓜被发现感染PRSV。本文于2012-2016年对转基因番木瓜样品进行了实地调查，并将二十个代表性分离株人工接种到西葫芦和‘华农1号’番木瓜中，研究发现植物表现出明显的病害症状。120个感染PRSV分离株的西葫芦基因的系统发育分析表明，PRSV可分为三个组。且分离株之间存在高度的遗传分化，27.1%的菌株根据CP核苷酸序列被鉴定为重组体。研究结果表明，PRSV的遗传变异和新病毒谱系的形成课表明转基因番木瓜在华南地区的抗性丧失。
外文摘要：In 2006, the release and cultivation of the genetically modified papaya cultivar 'Huanong No.1' successfully controlled the destructive papaya ringspot disease caused by Papaya ringspot virus (PRSV) in South China. However, some transgenic papaya plants from Guangdong and Hainan are found infected by PRSV. In this study, Field investigation was carried out and susceptible transgenic papaya samples were collected during 2012-2016. Twenty representative isolates were artificially inoculated into Cucurbita pepo and commercialised 'Huanong No. 1' papaya, and results indicated that the plants showed obvious disease symptoms. Phylogenetic analysis of CP genes of 120 PRSV-infected isolates showed that PRSV can be divided into three groups. Isolates from Guangdong and Hainan belong to Group III, which is further divided into two subgroups. The isolates collected in this study have greatly diverged from the previously reported dominant strains Ys, Vb and Sm in South China, indicating that they belong to a new lineage. Further analysis showed a highly genetic differentiation between isolates, and 27.1% of the isolates were identified as recombinants on the basis of CP nucleotide sequences. These results indicate that the genetic variation of PRSV and the formation of the new virus lineage may explain the loss of transgenic papaya resistance in South China.
作者：Wu, Zilin; Mo, Cuiping; Zhang, Shuguang; Li, Huaping
作者单位：South China Agr Univ
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.122
出版年份：2018
出版刊次：5
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