中文摘要：本文研究了一种以PAN/BAR蛋白为靶点的高灵敏度电化学发光（ECL）免疫检测技术，该技术科利用从玉米粉调色剂中制备的碳纳米颗粒（CNPs）对转基因油菜进行检测。作者利用CNPs与抗体的PAT / bar蛋白来修改工作电极。PAT／bar蛋白与其抗体之间发生免疫反应后，在电极受体区域上形成的免疫复合物会抑制电极表面和ECL物质之间的电子转移，从而导致ECL响应的降低。在最佳条件下，随着PAT／bar蛋白浓度的增加，转基因油菜RF3含量在0.10-10ng/ml和0.050-1.0%范围内呈线性下降，检测限为0.050 ng/ml和0.020%（S/N＝3）。本文的研究结果为农业转基因油菜的安全控制开辟了一条简便、灵敏、快速的途径。
外文摘要：A highly sensitive electrochemiluminescent (ECL) immunoassay targeting PAT/bar protein was facilely developed for genetically modified (GM) rapeseed detection using carbon nanoparticles (CNPs) originally prepared from printer toner. In this work, CNPs linked with antibody for PAT/bar protein were used to modify a working electrode. After an immunoreaction between the PAT/bar protein and its antibody, the immunocomplex formed on the electrode receptor region resulted in an inhibition of electron transfer between the electrode surface and the ECL substance, thus led to a decrease of ECL response. Under the optimal conditions, the ECL responses linearly decreased as the increase of the PAT/bar protein concentration and the GM rapeseed RF3 content in the ranges of 0.10-10 ng/mL and 0.050-1.0%, with the limits of detection of 0.050 ng/mL and 0.020% (S/N = 3). These results open a facile, sensitive, and rapid approach for the safety control of agricultural GM rape.
外文关键词：immunoassay, electrochemiluminescence, genetically modified rapeseed, PAT/bar protein, carbon nanoparticles
作者：Gao, Hongfei; Wen, Luke; Wu, Yuhua; Yang, Xiaohong; Li, Jun; Li, Xiaofei; Fu, Zhifeng; Wu, Gang
作者单位：Southwest Univ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：3.412
出版年份：2018
出版刊次：5
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