中文摘要：过氧化物酶纯化可以提高底物降解能力，本研究利用扩张床吸附（EBA）结合渗滤/超滤（DF/UF）对米糠中的过氧化物酶进行了纯化，并将其用于减少脱氧雪腐镰刀菌烯醇（DON）。结果显示，在模拟试验中，通过EBA与DF/UF联合纯化的过氧化物酶导致DON减少81.7%。因此，农副产品中纯化过氧物酶在降低DON水平方面具有广阔的应用前景。
外文摘要：BACKGROUND: Once peroxidase purification can improve substrate degradation capacity, peroxidase from rice bran was purified by expanded bed adsorption (EBA) coupled to diafiltration/ultrafiltration (DF/UF) and applied for deoxynivalenol (DON) reduction.
RESULTS: The DF/UF step was optimized according to membrane molecular weight cutoff (MWCO), DF cycles number, operation temperature and CaCl2 addition to the DF buffer. DON reduction tests were carried out with both clarified and purified peroxidase. The use of MWCO of 10 kDa, 10 degrees C, six DF cycles and adding 4 m mol L-1 CaCl2 to DF buffer promoted enzyme purification of 4.4-fold and enzymatic recovery of 79.4%. The global purification process combining two techniques resulted in enzyme purification of 75.1-fold with an enzymatic recovery of 22.8%. Peroxidase purified by EBA combined with DF/UF resulted in DON reduction of 81.7% in a model system.
CONCLUSION: Peroxidase purification was achieved by scale-up integrated strategies. The use of purified peroxidase from agricultural by-products could be a promising alternative for enzymatic DON levels reduction.
 
外文关键词：bioremediation; agricultural by-product; expanded bed adsorption; mycotoxin; oxidoreductases; ultrafiltration
作者：Gauterio, Gabrielle Victoria；Malta, Danielle Specht；Reginatto, Lais；等
作者单位：Universidade Federal do Rio Grande
期刊名称：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
期刊影响因子：2.587
出版年份：2017
出版刊次：8
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