中文摘要：在环境中检测、监测和定位转基因序列一直是生态学中的一个长期挑战。在表观水平上转基因DNA的真实映射需要一种DNA扩增的方法，该方法应具有鲁棒性和等温性，以便具有成本效益和进行田间试验。本文提出了一种具有这些特性的方法。多重荧光法用于检测在转基因植物中经常使用的P35S CAMV启动子以及特异于玉米的α-玉米蛋白，但也可以适用于任何其他DNA序列。该方法使用环介导的等温扩增技术及DNA杂交探针的荧光团猝灭系统实现可视化。本文证实了这种三色方法多重荧光、等温方法适用于转基因玉米、非转基因玉米和非转基因/非玉米品种的检测。
外文摘要：Detecting, monitoring, and mapping transgenic sequences in the environment has been a long-standing challenge in ecology. Given the level of social, economic, and scientific interest in them, transgenic (GM) DNA sequences stand as paradigmatic; such sequences further have the unique property of functioning as unequivocal markers in the environment, clearly showing the paths of gene flow between crops and their wild relatives, and across industrial and traditional forms of agriculture. True mapping of transgenic DNA at the landscape level requires a method of DNA amplification that is robust and isothermal in order to be cost effective and field-based. We present here a method with these characteristics. Our multiplex fluorescent method is for the detection of the p35s CaMV promoter frequently used in transgenic plants as well as the alpha-zein protein specific to maize, but can be applicable to any other DNA sequence. The method uses loop-mediated isothermal amplification (LAMP) with visualization achieved using a fluorophore-quencher system of DNA hybridizing probes. We demonstrate the applicability of this tri-color method to transgenic corn, non-transgenic corn, and non-transgenic/non-corn species.
 
外文关键词：LAMP, Isothermal DNA amplification, GMOs, Maize, Field detection, Multiplex LAMP, Fluorescent LAMP
作者：Bektas, Ali
作者单位：Univ Calif Berkeley
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.245
出版年份：2018
出版刊次：5
点击下载：转基因玉米的多重荧光、等温检测方法研究