中文摘要：转基因大豆在传统种子库中的持续存在已成为国际种子贸易中日益严重的问题。鉴于此，种子公司推动了对精确转基因大豆种子检测的常规试验的发展。本文利用标准化PCR方法检测和量化非转基因大豆样品中的转基因种子该方法利用靶向CP-4烯醇丙酮酸莽草酸-3-磷酸合成酶（CP4 EPSPS）基因的P35S F2/PETU-R1引物，通过MelIc QuANT GMO检测试验进行实时PCR检测和定量。研究发现，该方法能有效地检测和定量一批大豆种子中转基因种子中的存在。本研究中所描述的敏感转基因定量方法将为监管活动提供支持，并有助于转基因大豆的加工和控制。
外文摘要： The constant presence of genetically modified (GM) soybean in conventional seed lots has become a growing problem for international seed trade. In this context, seed companies have prompted the development of routine tests for accurate genetically modified soybean seeds detection. In this study, a quantitative PCR-based method was standardized in order to detect and quantify mixtures of seeds (i.e. certified seed) or GM grains (i.e. seeds came from field) into samples of non-GM soybean, in a way that soybean lots can be assessed within the standards established by legislation. The method involved the use of p35S-f2/petu-r1 primers targeting CP-4 enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) gene (i.e. that confers herbicide tolerance in Roundup Ready (TM) (RR)) for real-time PCR detection and quantification through mericon Quant GMO Detection Assay. The results revealed the method efficiency to detect and quantify the presence of even one soybean seed in batch used for routine evaluation of GM seeds. In addition, it was possible to detect of up to 0.1% of transgenic DNA relative to the soybean grains content. Thus, the sensitive GMO quantitative approach described in this study will provide support in supervising activities, and facilitate the process and control of GM soybean.
外文关键词：Roundup Ready (R) soybean, GMP, Glycine max, Cp4-epsps gene
作者： Leao-Buchir, Joelma; Melo Pereira, Gilberto Vinicius; Lopes da Silva, Andre Luis; Alban, Silvana; Rocha, Maria Carolina; Polettini, Jossimara; Thomaz-Soccol, Vanete; Soccol, Carlos Ricardo
作者单位：Univ Fed Parana
期刊名称：BIOSCIENCE JOURNAL
期刊影响因子：2.015
出版年份：2018
出版刊次：2