用高速逆流色谱分级洗脱从海洋镰刀菌LS68中制备分离纯化单端孢霉烯族毒素

Preparative Separation and Purification of Trichothecene Mycotoxins from the Marine Fungus Fusarium sp LS68 by High-Speed Countercurrent Chromatography in Stepwise Elution Mode

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中文摘要:单端孢霉烯族毒素污染食品和动物饲料是一个人类和动物健康日益关注的问题。大量的单端孢霉烯族毒素是食品安全监测和毒理学研究所必需的。本研究描述了一种新型的从海洋真菌,镰刀菌LS68中纯化单端孢霉烯族毒素的有效方法-高速逆流色谱分级洗脱(HSCCC)法。研究表明,HSCCC法能有效分离单端孢霉烯族毒素,用于制定纯商业标准和研究标准。
外文摘要:The contamination of foods and animal feeds with trichothecene mycotoxins is a growing concern for human and animal health. As such, large quantities of pure trichothecene mycotoxins are necessary for food safety monitoring and toxicological research. A new and effective method for the purification of trichothecene mycotoxins from a marine fungus, Fusarium sp. LS68, is described herein. Preparative high-speed countercurrent chromatography (HSCCC) was utilized for the scalable isolation and purification of four trichothecene mycotoxins for the first time in stepwise elution mode, with a biphasic solvent system composed of hexanes-EtOAc-CH₃OH-H₂O (6:4:5:5, v/v/v/v) and (8.5:1.5:5:5,v/v/v/v). This preparative HSCCC separation was performed on 200 mg of crude sample to yield four trichothecene mycotoxins, roridin E (1), roridin E acetate (2), verrucarin L acetate (3), and verrucarin J (4) in a single run, with each of >98% purity. These compounds were identified by MS, ¹H NMR, 13C NMR, and polarimetry. The results demonstrate an efficient HSCCC method for the separation of trichothecene mycotoxins, which can be utilized to produce pure commercial and research standards.
外文关键词:trichothecene mycotoxins;roridin;verrucarin;high-speed countercurrent chromatography;preparative separation;stepwise elution
作者:Liu, Y;Zhou, XZ;Naman, CB;Lu, YB;Ding, LJ;He, S
作者单位:Ningbo Univ
期刊名称:MARINE DRUGS
期刊影响因子:3.345
出版年份:2018
出版刊次:2
点击下载:用高速逆流色谱分级洗脱从海洋镰刀菌LS68中制备分离纯化单端孢霉烯族毒素
  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2018-07-25