中文摘要：B-9菌株，与Sphingosinicella microcystinivorans菌株Y2的相似率为99%，是革兰氏阴性菌，能用来降解微囊藻毒素相关化合物和节球藻毒素。本研究拟拓宽B-9菌株的应用领域，将它应用于降解真菌产生的霉菌毒素。研究表明B-9菌株包含一个可命名为MlrE的水解酶，以及一个酯酶。
外文摘要：Strain B-9, which has a 99% similarity to Sphingosinicella microcystinivorans strain Y2, is a Gram-negative bacterium with potential for use in the degradation of microcystin-related compounds and nodularin. We attempted to extend the application area of strain B-9 and applied it to mycotoxins produced by fungi. Among the tested mycotoxins, only ochratoxin A was completely hydrolyzed to provide the constituents ochratoxin α and l-phenylalanine, and levels of fumonisin B1 gradually decreased after 96 h. However, although drugs including antibiotics released into the aquatic environment were applied for microbial degradation using strain B-9, no degradation occurred. These results suggest that strain B-9 can only degrade amino acid-containing compounds. As expected, the tested compounds with amide and ester bonds, such as 3,4-dimethyl hippuric acid and 4-benzyl aspartate, were readily hydrolyzed by strain B-9, although the sulfonamides remained unchanged. The ester compounds were characteristically and rapidly hydrolyzed as soon as they came into contact with strain B-9. Furthermore, the degradation of amide and ester compounds with amino acids was not inhibited by the addition of ethylenediaminetetraacetic acid (EDTA), indicating that the responsible enzyme was not MlrC. These results suggest that strain B-9 possesses an additional hydrolytic enzyme that should be designated as MlrE, as well as an esterase.
外文关键词：microcystin-degrading bacteria；mycotoxin；protease；esterase；inhibitor
作者：Jin, HY；Hiraoka, Y；Okuma, Y；Hashimoto, EH；Kurita, M；Anas, ARJ；Uemura, H；Tsuji, K；Harada, KI
作者单位：Meijo Univ
期刊名称：MARINE DRUGS
期刊影响因子：3.345
出版年份：2018
出版刊次：2
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