中文摘要：本研究开发了一种基于适体的侧流试纸条，用于检测玉米赤霉烯酮（ZEN）。在最佳实验条件下，ZEN的检测范围为5-200ng/ml，检测限为20ng/ml。该测试条已成功应用于玉米中ZEN的测定，回收率为93.4%-114.2%，所有检测可在5分钟内完成。表明本研究开发的基于适体的侧流试纸条可用于快速、灵敏地检测ZEN。
外文摘要：An aptamer-based lateral flow test strip was developed for the detection of zearalenone (ZEN). This assay was based on the competition for the aptamer between ZEN and its complementary sequence. Several experimental conditions that could influence sensitivity have been investigated, including the concentration of aptamer and NaCl used in the probe preparation, the mole ratio of streptavidin and biotinylated DNA used in the preparation of test line and control line, and the loading quantity of gold nanoparticles-aptamer conjugates (AuNPs-Apt). Under the optimal experimental conditions, we successfully detected ZEN within a detection range of 5-200 ng/mL and the visual limit of detection of 20 ng/mL. This aptamer-based strip was successfully applied to the determination of ZEN in spiked corn samples, and the recoveries were from 93.4% to 114.2%. All detections can be achieved within 5 min. The results demonstrated that the developed aptamer-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of ZEN.
外文关键词：lateral flow test strip；aptamer；zearalenone；gold nanoparticles
作者：Wu, Shijia；Liu, Lihong；Duan, Nuo等.
作者单位：Jiangnan University
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：3.412
出版年份：2018
出版刊次：8
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