中文摘要：本研究开发了一种简单的检测玉米中的串珠镰刀菌素（MON）的LC-MS/MS法，并应用于食品监管部门的样品检测分析。试验证实，与其他测试溶剂相比，用乙腈/水（50/50（v/v））萃取均质样品的效率最高。试验采用乙腈稀释离心提取物，通过HILLC（疏水相互作用液相色谱）-HPLC层析分离后进行测定，不需要任何净化（稀释和注射方法）。该方法的LOD和LQD分别达到了2.6μg/kg和2.8μg/kg。研究于2014-2015从在德国巴伐利亚市场的工厂、电影院、批发和零售店收集了39份爆米花、玉米粉和粗面粉样品，检测结果表明：MON的污染率高达97%，污染水平介于LOD-847μg/kg之间，平均水平为118μg/kg，中位数为39μg/kg，在玉米粉中检出了最高值。
外文摘要：A simple LC-MS/MS method was developed for the determination of moniliformin (MON) in maize and applied for the analysis of samples within the official food surveillance. The homogenized samples were extracted with acetonitrile/water 50/50 (v/v) which proved to have the highest extraction efficiency compared to other tested solvents. The centrifuged extracts were diluted with acetonitrile and were measured after chromatographic separation by HILIC (hydrophobic interaction liquid chromatography)-HPLC without any cleanup (dilute and shoot approach). The LOD and LOQ achieved by this procedure were 2.6 and 8.8 mu g/kg, respectively. Thirty-nine samples of popcorn, maize meal, and semolina were collected in 2014 and 2015 at mills, cinemas, wholesale, and retail from the Bavarian market (Germany). The rate of contamination with MON was very high (97%) with levels ranging between the LOD and 847 mu g/kg. The mean level was 118 mu g/kg and the median, 39 mu g/kg. The maximum value was detected in maize meal. The results are discussed with respect to possible health implications for the consumer.
外文关键词：Maize；Mycotoxins；Moniliformin；LC-MS/MS HILIC
作者：Barthel, Joerg；Rapp, Martin；Holtmannspoetter, Heinrich等.
作者单位：Bavarian Health & Food Safety Authority
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.854
出版年份：2018
出版刊次：1
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