中文摘要：本研究在水相中合成CdTe/CdS/ZnS量子点(QDs)，并将其与一种新的抗玉米赤霉烯酮(ZEN)单克隆抗体结合。用这种新型荧光探针，研制了一种荧光免疫分析法(FLISA)及一种快速免疫层析试纸条(ICTS)，用来检测玉米中的ZEN。结果证实，一，用FLISA检测ZEN，稳健而灵敏；二，理论上ICTS非常适合于快速筛查大量样品。
外文摘要：CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038-0.977 ng/mL with an IC50 of 0.162 ng/mL and a limit of detection (LOD) of 0.012 ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0 ng/mL in buffer solution and 1.5 ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.
外文关键词：Zearalenone；Quantum dots；CdTe/CdS/ZnS；Fluoroimmunoassay；Immunochromatographic strip
作者：Zhang, FY；Liu, B；Sheng, W；Zhang, Y；Liu, Q；Li, SJ；Wang, S
作者单位：Tianjin Univ Sci & Technol
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2018
出版刊次：7
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