中文摘要：本研究旨在评估热灭活面包酵母(HKBY)、面包酵母细胞壁(CWBY)及面包酵母细胞壁(1,3)-β-D葡聚糖(BGBY) 在添加了0.5μg/mL AFB1的磷酸盐缓冲液(PBS)中结合AFB1的能力。结果表明BGBY粘合剂的效果最好，且结合时间越长，从PBS中去除的AFB1越多。
外文摘要：The aim of this study was to evaluate the ability of heat-killed baker's yeast (HKBY), the cell wall of baker's yeast (CWBY), and cell wall (1 -> 3)-beta-D-glucan of baker's yeast (BGBY) to bind aflatoxin B-1 (AFB(1)) in phosphate-buffered saline (PBS) spiked with 0.5 mu g/mL AFB(1). Baker's yeast (Saccharomyces cerevisiae) was heat killed by autoclaving at 121 degrees C for 10 min. The cell wall was physically extracted, and (1 -> 3)-beta-D-glucan was extracted by a modified method. The concentration of AFB(1) was determined by high-performance liquid chromatography after exposure to binders for three contact times, 30 min, 5 h, and 24 h, at room temperature. AFB(1) binding by HKBY, CWBY, and BGBY was 6.30 to 46.34%. The lowest binding capacity was found for HKBY with a contact time of 30 min, and the highest binding capacity was found for BGBY with a contact time of 24 h. Among binders, CWBY had the highest binder-AFB(1) complex stability during washing with PBS, and the lowest stability was found for HKBY complexes. Results of this study indicated that BGBY was the most effective binder, and more exposure to BGBY removes more AFB(1) from PBS.
外文关键词：Aflatoxin B-1；Baker's yeast；Binding capacity；Cell wall；(1 -> 3)-beta-D-Glucan
作者：Aazami, MH；Nasri, MHF；Mojtahedi, M；Mohammadi, SR
作者单位：Univ Birjand
期刊名称：JOURNAL OF FOOD PROTECTION
期刊影响因子：1.609
出版年份：2018
出版刊次：4
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