中文摘要：Bt作物技术对非靶标节肢动物潜在影响的田间研究可以补充实验室研究的数据以支持环境风险评估。本文在巴西进行了三年的实地研究，以评估产生CYA1AC和CYL1F蛋白的DAS-81419-2大豆的潜在影响。本文在巴西三个大豆产区研究了转Bt基因大豆和非转基因大豆在喷施或未喷施杀虫剂的条件下对其非靶标节肢动物多样性和丰度的影响。作者利用莫里克陷阱和陷阱诱捕法调查非目标节肢动物丰度，并计算每个节肢动物样品在不同生长阶段和杀虫剂处理下的总丰度（N）、丰度（S）、香农维纳指数（H）、辛普森指数（D）和皮卢均匀度（J）。采用FAN分析法选择最有代表性的NTAS来描述与大豆相关的NTA群落结构，并通过主响应曲线方法来检验由于杀虫剂处理效果所产生的影响。研究发现，与未喷施杀虫剂的非转基因大豆相比，转Bt基因大豆对其非靶标节肢动物丰度和多样性的影响不明显。研究结果表明，产生CYC1AC和CYL1F毒素的转基因大豆DAS-81419-2不影响与其相关的其非靶标节肢动物群落。
外文摘要：Field-scale studies that examine the potential for adverse effects of Bt crop technology on non-target arthropods may supplement data from laboratory studies to support an environmental risk assessment. A three year field study was conducted in Brazil to evaluate potential for adverse effects of cultivating soybean event DAS-81419-2 that produces the Cry1Ac and Cry1F proteins. To do so, we examined the diversity and abundance of non-target arthropods (NTAs) in Bt soybean in comparison with its non-Bt near isoline, with and without conventional insecticide applications, in three Brazilian soybean producing regions. Non-target arthropod abundance was surveyed using Moericke traps (yellow pan) and pitfall trapping. Total abundance (N), richness (S), Shannon-Wiener (H'), Simpson's (D) and Pielou's evenness (J) values for arthropod samples were calculated for each treatment and sampling period (soybean growth stages). A faunistic analysis was used to select the most representative NTAs which were used to describe the NTA community structure associated with soybean, and to test for effects due to the treatments effects via application of the Principal Response Curve (PRC) method. Across all years and sites, a total of 254,054 individuals from 190 taxa were collected by Moericke traps, while 29,813 individuals from 100 taxa were collected using pitfall traps. Across sites and sampling dates, the abundance and diversity measurements of representative NTAs were not significantly affected by Bt soybean as compared with non-sprayed non-Bt soybean. Similarly, community analyses and repeated measures ANOVA, when applicable, indicated that neither Bt soybean nor insecticide sprays altered the structure of the NTA communities under study. These results support the conclusion that transgenic soybean event DAS-81419-2 producing Cry1Ac and Cry1F toxins does not adversely affect the NTA community associated with soybean.
作者：Marques, Luiz H.; Santos, Antonio C.; Castro, Boris A.; Storer, Nicholas P.; Babcock, Jonathan M.; Lepping, Miles D.; Sa, Verissimo; Moscardini, Valeria F.; Rule, Dwain M.; Fernandes, Odair A.
作者单位：Dow AgroSci Ind Ltda
期刊名称：PLOS ONE
期刊影响因子：2.766
出版年份：2018
出版刊次：2
点击下载：巴西转CYC1AC和CYL1F基因大豆对其非靶标节肢动物群落的影响研究