中文摘要：由于真菌共存，农产品监测中频频发现多种霉菌毒素污染。但是，许多检测方法只针对单一霉菌毒素，凸显了在一次运行中对多种真菌毒素进行现场测定的需求。本研究研制了一种多色免疫层析法(ICS)，用来同时检测玉米及动物饲料中的黄曲霉毒素B1 (AFB1)、玉米赤霉烯酮(ZEN)及T-2毒素。研究表明，该多色ICS法具有灵敏、快速视觉分辨的特点，能在20分钟内同时半定量测定玉米及谷物饲料中的黄曲霉毒素B1、玉米赤霉烯酮及T-2毒素。
外文摘要：Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-based feed samples within 20 min.
外文关键词：multicolor；immunochromatographic assay；aflatoxin B1；zearalenone；T-2
作者：Xu, L；Zhang, ZW；Zhang, Q；Zhang, W；Yu, L；Wang, D；Li, H；Li, PW
作者单位：Chinese Acad Agr Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：2
点击下载：多色免疫层析法现场同时半定量测定玉米及谷物饲料中的黄曲霉毒素B1、玉米赤霉烯酮及T-2毒素