中文摘要：镰刀菌玉米赤霉烯酮（ZEA）化学型的早期检测可能是一种预防ZEA污染大米的措施。本研究利用四个引物对ZEA生物合成基因PKS3、PKS13、ZEB1及ZEB2进行定位，建立了一种多重聚合酶链式反应(mPCR)分析，用来检测大米中产ZEA的真菌。采用了两种mPCR法：其一是将分离的镰刀菌DNA扩增（常规方法），其二是直接扩增大米的目标DNA而无需耗时的DNA分离（直接法）。研究表明，两种mPCR法均适用于精米和糙米中产ZEA镰刀菌的检测；而直接法更快捷。
外文摘要：Early detection of the zearalenone (ZEA) chemotype of Fusarium species could be a precautionary measure for preventing ZEA contamination in rice. In this study, a multiplex polymerase chain reaction (mPCR) assay for detecting ZEA-producing fungi in rice was established using a set of four primers targeting the ZEA biosynthesis genes PKS3, PKS13, ZEB1, and ZEB2. Two mPCR approaches were used: one that amplified the DNA obtained from Fusarium isolates (conventional method) and another that directly amplified the target DNA from rice samples without time-consuming DNA isolation (direct method). The two mPCR methods showed high sensitivity in detecting ZEA-producing species, with a detection limit of 1.25 pg/mu L. of genomic DNA and 102 and 103 spores/g of white and brown rice, respectively. Both methods were specific for ZEA-producing species and gave four band patterns. The application of the two mPCR methods to 51 Fusarium isolates and 41 rice samples revealed that 31% (16 of 51) and 24% (10 of 41) of the samples were contaminated with ZEA-producing species, respectively. The mPCR results were further evaluated using high-performance liquid chromatography; in general, the two methods yielded similar results. These findings indicate that both mPCR methods are suitable for the detection of ZEA-producing Fusarium species in white and brown rice; however, the direct method yielded more rapid results.
外文关键词：Fusarium species；Zearalenone chemotype；Multiplex PCR；Conventional and direct methods；Rice
作者：Sim, JH；Tian, F；Jung, SY；Auh, JH；Chun, HS
作者单位：Chung Ang Univ
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.445
出版年份：2018
出版刊次：3
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