中文摘要：本研究研制了一种灵敏而特异性的超高效液相色谱-串联质谱联用(UPLC-MS/MS)法，用来定量测定肉鸡血浆中的伏马菌素B1 (FB1)及其部分水解代谢物pHFB1a+b和水解HFB1，以及伏马菌素B2（FB2）。该方法已成功用于肉鸡HFB1的毒物动力学和生物转化的研究。
外文摘要：A sensitive and specific method for the quantitative determination of Fumonisin B1 (FB1), its partially hydrolysed metabolites pHFB1a+b and hydrolysed metabolite HFB1, and Fumonisin B2 (FB2) in broiler chicken plasma using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS) was developed. The sample preparation was rapid, straightforward and consisted of a deproteinization and phospholipid removal step using an Oasis((R)) Ostro(TM) 96-well plate. Chromatography was performed on an Acquity HSS-T3 column, using 0.3% formic acid and 10 mM ammonium formate in water, and acetonitrile as mobile phases. The MS/MS instrument was operated in the positive electrospray ionization mode and the two multiple reaction monitoring transitions were monitored for each component for quantification and identification, respectively. The method was validated in-house: matrix-matched calibration graphs were prepared and good linearity (r 0.99) was achieved over the concentration ranges tested (1-500 ng/mL for FB1 and FB2; 0.86-860 ng/mL for pHFB1a; 0.72-1430 ng/mL for pHFB1b and 2.5-2500 ng/mL for HFB1). Limits of quantification (LOQ) and detection (LOD) in plasma ranged between 0.72 to 2.5 ng/mL and 0.03 to 0.17 ng/mL, respectively. The results for the within-day and between-day precision and accuracy fell within the specified ranges. Moreover, the method was transferred to an UPLC high-resolution mass spectrometry (HR-MS) instrument in order to determine potential metabolites of HFB1, such as N-acyl-HFB1s and phase II metabolites. The method has been successfully applied to investigate the toxicokinetics and biotransformation of HFB1 in broiler chickens.
外文关键词：fumonisin B1；hydrolysed FB1；fumonisin B2；UPLC-MS；MS；UPLC-HR-MS；plasma；broiler chickens；toxicokinetics
作者：De Baere, S；Croubels, S；Novak, B；ichl, G；Antonissen, G
作者单位：Dept Pharmacol Toxicol & Biochem
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：2
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