中文摘要：本文对大鼠进行为期90天的喂养试验，以对转基因大豆DAS-81419-2的亚慢性口服毒性进行了评价。作者对大鼠分别饲喂含有30%、15%、7.5%或0%的转基因大豆DAS-81419-2与常规大豆豆粕的饲料。在实验中，观察大鼠的行为、体重和食物消耗情况；在实验的中期和末期，收集大鼠的血液、血清和尿液样本进行生化分析；在实验结束时，对大鼠的内脏器官进行称重并完成组织病理学检查。研究发现，转基因大豆组和常规大豆组中大鼠的活动均自由，且没有任何异常症状或异常分泌物在他们的鼻子、眼睛或嘴周围；大鼠体重均无不良影响；未观察到血液学、生化或尿液指标的生物学差异；相对脏器重量无显著差异；未观察到组织病理学改变。研究结果表明，转基因大豆DAS-81419-2对大鼠为期90天的饲喂实验，没有引起大鼠任何与治疗相关的影响。
外文摘要：A 90-day feeding study in rats was conducted to evaluate the subchronic oral toxicity of genetically modified (GM) DAS-81419-2 soybean. Wistar rats were fed with diets containing toasted soybean meal produced from DAS-81419-2 soybean grain that expresses the Cry1F, Cry1Ac, and Pat proteins or containing conventional soybean at doses of 30.0%, 15.0%, 7.5%, or 0% (control group) for 90 consecutive days. The general behavior, body weight and food consumption were observed. At the middle and end of the experiment, blood, serum, and urine samples were collected for biochemical assays. At the conclusion of the study, the internal organs were weighed and histopathological examination was completed. The rats exhibited free movement and shiny coats without any abnormal symptoms or abnormal secretions in their noses, eyes, or mouths. There were no adverse effects on body weight in GM soybean groups and conventional soybean groups. No biological differences in hematological, biochemical, or urine indices were observed. No significant differences in relative organ weights were detected between the experimental groups and the control group. No histopathological changes were observed. Under the conditions of this study, DAS-81419-2 soybean did not cause any treatment-related effects in Wistar rats following 90 days of dietary administration.
外文关键词：Genetically modified soybean, cry1F gene, cry1Ac gene, 90-Day feeding study, Subchronic toxicity, Rat
作者：Qian, Zhi-Yong; Zhang, Shu-Jing; Zhang, Li; Zhang, Jing; Liu, Ying-Hua; Zhou, Qing-Hong; Jiang, Shu-Qing; Li, Shu-Fei
作者单位：Tianjin Ctr Dis Control & Prevent
期刊名称：REGULATORY TOXICOLOGY AND PHARMACOLOGY
期刊影响因子：2.815
出版年份：2018
出版刊次：7
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