中文摘要：转基因水稻在中国的商业发布是一个有争议的话题。虽然一系列的研究都集中在农业生态系统的安全性评价上，但转基因水稻的内生菌很少被考虑。本文对转基因水稻和非转基因水稻内生菌种群和群落的影响进行了比较研究。群体水平调查表明，Cy1Ab基因插入对水稻内生真菌的苗期有不同程度的影响，但在转基因水稻和野生稻之间仅观察到Zhejiang22叶片存在显著差异。利用16S rRNA基因的群落水平分析表明，在三个转基因水稻植株及其对应的亲本中，PyLA变形杆菌和厚壁菌门是主要的类群。与对照组相比，Minghui63和Xiushui11的内生群落对CYL1AB基因插入的反应弱于Zhejiang22，群落结果与群体调查结果一致。研究结果表明，水稻内生菌的群体和群落在不同水稻品种和植物组织中受到不同程度的Cys1Ab基因的影响。
外文摘要：The commercial release of genetically modified (GMO) rice for insect control in China is a subject of debate. Although a series of studies have focused on the safety evaluation of the agroecosystem, the endophytes of transgenic rice are rarely considered. Here, the influence of endophyte populations and communities was investigated and compared for transgenic and nontransgenic rice. Population-level investigation suggested that cry1Ab gene insertion influenced to a varying degree the rice endophytes at the seedling stage, but a significant difference was only observed in leaves of Bt22 (Zhejiang22 transgenic rice) between the GMO and wild-type rice. Community-level analysis using the 16S rRNA gene showed that strains of the phyla Proteobacteria and Firmicutes were the predominant groups occurring in the three transgenic rice plants and their corresponding parents. By contrast, the endophytic communities of Minghui63 and Xiushui11 showed a weaker response to cry1Ab gene insertion than did Zhejiang22, and the community results were consistent with the population-level investigation. The populations and communities of rice endophytes were affected by the cry1Ab gene to a different extent in different rice varieties and plant tissues. The results of this study broaden our understanding of unexpected transgenic influences on nontarget organisms.
外文关键词： 16S rRNA, community structure, endophytes, transgenic rice
作者：Wang, Xu; Cai, Mengyu; Zhou, Yu
作者单位：Anhui Agr Univ
期刊名称：TURKISH JOURNAL OF BIOLOGY
期刊影响因子：0.651
出版年份：2018
出版刊次：7
点击下载：Cy1Ab基因插入对转基因水稻内生细菌群落的生物学影响