中文摘要：研发同时检测两种高度关注的真菌毒素，赭曲霉毒素A(OTA)和黄曲霉毒素B1 (AFB1)的有效方法对食品安全监测具有重要意义。本研究研发了一种同时检测OTA和AFB1的磁控荧光传感器。研究发现，该简便传感方案操作简便、耗时短、选择性及灵敏度高，为双重霉菌毒素的高通量筛查提供了新的途径。
外文摘要：Development of an efficient method for the simultaneous detection of two highly concerning mycotoxins, ochratoxin A (OTA) and aflatoxin B1 (AFB1), is of great significance on food safety monitoring. Herein, a magnetically controlled fluorescence aptasensor for simultaneous determination of OTA and AFB1 has been successfully developed. The working principle of the aptasensor is based on the specific aptamer-mycotoxin recognition and further leads to the partial release of two distinguishable fluorescence labels from the magnetic carriers. Through the magnetic separation, the reporter probes in the supernatant solution can be collected and converted into a sensitive fluorescence signal with dual emission peaks. This aptasensor provided a wide detection range of 2 pg mL−1 − 5 ng mL−1 for OTA and 5 pg mL−1 − 10 ng mL−1 for AFB1. The new easy-to-wash and simple-to-use approach offers a simultaneous and high selective detection with high sensitivity (limits of detection of 0.67 and 1.70 pg mL−1 for OTA and AFB1, respectively). Remarkable accuracy (relative standard deviation < 5.6%) during the mycotoxins determination as well as excellent quantitative recoveries (95–108%) during the analysis of the spiked corn samples were also achieved. This simple aptasensing scheme provides a new avenue for high throughput screen of dual mycotoxins due to its simple manipulation, short assay times, high selectivity and sensitivity.
外文关键词：Fluorescence aptasensor；Magnetic separation；Simultaneous determination；Ochratoxin A；Aflatoxins B1
作者：Qian, J；Ren, CC；Wang, CQ；Chen, W；Lu, XT；Li, HA；Liu, Q；Hao, N；Li, HM；Wang, K
作者单位：Jiangsu Univ
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：4.712
出版年份：2018
出版刊次：8
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