中文摘要：本研究研制了一种液液萃取-固相萃取-超高效液相色谱-串联质谱联用(UPLC-MS/MS)分析法，用来同时检测花生油中的黄曲霉毒素B1 (AFB1)、双酚A (BPA)及4-壬基酚(4-NP)。并用该方法成功分析了52个花生油样品，在其中43个样品了检出了AFB1和4-NP，浓度范围分别为0.5–69.4和9.3–77.8 μg/kg；所有样品中均未检出BPA。
外文摘要：An analytical method based on liquid-liquid extraction combined with solid-phase extraction and isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was well developed for simultaneous determination of aflatoxin B1 (AFB1), bisphenol A (BPA), and 4-nonylphenol (4-NP) in peanut oil. After adding isotope internal standards, the samples were firstly diluted by normal hexane and then extracted by acetonitrile and Carb/PSA solid-phase extraction cartridge in sequence to obtain the extracted solution. All the extracted solution was merged and was subsequently dried to near dryness by a mild nitrogen stream. Three target analytes were separated on a Phenomenex Luna C18 chromatographic column, quantified by an internal standard method and detected by ESI positive (ESI+) and negative (ESI−) subsection acquisition modes under multi-reaction monitoring (MRM) conditions. Results demonstrated that the three target analytes exhibited excellent linearity in their corresponding concentration ranges of 0.1–100.0 μg/L with correlation coefficients all greater than 0.998. The corresponding method limits of quantitation (MLOQ, S/N = 10) of AFB1, BPA, and 4-NP were 0.2, 1.0, and 2.0 μg/kg, respectively. Moreover, the mean recoveries for negative samples spiked at three concentration levels were calculated between 87.7 and 105.1% with relative standard deviation (RSD, n = 6) ranging from 2.2 to 7.9% and the interday precision (n = 5) ranging from 5.0 to 8.7%. Finally, the method was successfully applied to analyze 52 peanut oil samples, and AFB1 and 4-NP were detected in 43 samples with the concentrations in the ranges of 0.5–69.4 and 9.3–77.8 μg/kg, respectively. None of BPA was detected in any samples.
外文关键词：Peanut oil；Aflatoxin B1；Bisphenol A；4-Nonylphenol；Solid-phase extraction；Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
作者：Deng, HL；Su, XG；Wang, HB
作者单位：Guangdong Food & Drug Vocat Coll
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：5
点击下载：通过液液萃取-固相萃取-超高效液相色谱-串联质谱联用同时检测花生油中的黄曲霉毒素B1、双酚A及4-壬基酚