中文摘要：由于在升高的CO2下生长的Bt作物中外源毒素含量的普遍减少，Bt作物将面临降低对靶标害虫有效性的新生态风险。选择处理这一问题的方法可能会影响转基因作物作为一种有效害虫管理工具的可持续性，特别是在未来大气CO2水平升高的情况下。本文选择根际细菌作为提高作物氮素利用率的潜在生物调节剂，于2016-2017年在开放顶室环境和双环境CO2下，研究了浸染Azospirillum brasilense（AB）和Azotobacter chroococcum（AC）的Bt玉米（IE09S034与其非转基因Xianyu 335亲本）对其靶标粘虫发育和食物利用的影响。研究发现，根际细菌感染显著地提高了分离幼虫的寿命、蛹期、相对消耗率和近似消化率，显著化蛹率、蛹重、成虫寿命、繁殖力、相对生长量、消化食物转化效率、摄入食物转化效率。CO2浓度升高和Bt玉米对玉米分离株的生长发育和食物利用都有负面影响。
外文摘要：Background: Bt crops will face a new ecological risk of reduced effectiveness against target-insect pests owing to the general decrease in exogenous-toxin content in Bt crops grown under elevated carbon dioxide (CO2). The method chosen to deal with this issue may affect the sustainability of transgenic crops as an effective pest management tool, especially under future atmospheric CO2 level raising. Methods: In this study, rhizobacterias, as being one potential biological regulator to enhance nitrogen utilization efficiency of crops, was selected and the effects of Bt maize (Line IE09S034 with Cry1Ie vs. its parental line of non-Bt maize Xianyu 335) infected by Azospirillum brasilense (AB) and Azotobacter chroococcum (AC) on the development and food utilization of the target Mythimna separate under ambient and double-ambient CO2 in open-top chambers from 2016 to 2017. Results: The results indicated that rhizobacteria infection significantly increased the larval life-span, pupal duration, relative consumption rate and approximate digestibility of M. separata, and significantly decreased the pupation rate, pupal weight, adult longevity, fecundity, relative growth rate, efficiency of conversion of digested food and efficiency of conversion of ingested food of M. separata fed on Bt maize, while here were opposite trends in development and food utilization of M. separata fed on non-Bt maize infected with AB and AC compared with the control buffer in 2016 and 2017 regardless of CO2 level. Discussion: Simultaneously, elevated CO2 and Bt maize both had negative influence on the development and food utilization of M. separata. Presumably, CO2 concentration arising in future significantly can increase their intake of food and harm to maize crop; however, Bt maize infected with rhizobacterias can reduce the field hazards from M. separata and the application of rhizobacteria infection can enhance the resistance of Bt maize against target lepidoptera pests especially under elevated CO2.
外文关键词：Elevated CO2, Growth and development, Transgenic Bt maize, Food utilization, Rhizobacteria, Mythimna separata
作者：Li, Zhuo; Parajulee, Megha N.; Chen, Fajun
作者单位：Nanjing Agr Univ
期刊名称：PEERJ
期刊影响因子：2.118
出版年份：2018
出版刊次：7
点击下载：CO2升高对饲喂转基因Bt玉米（经固氮菌侵染）的粘虫发育和食物利用的影响研究