中文摘要：本文描述了一种简便而精确的同时测定精米及米糠中两种共同发生的镰刀菌毒素，雪腐镰刀菌烯醇(NIV)和脱氧雪腐镰刀菌烯醇(DON)的方法。该方法包括运用免疫亲和柱纯化及HPLC-UV定量测定。测定结果表明，基于发生数据估算的韩国人NIV和DON的膳食摄入量远低于所建立的可耐受日摄入量。
外文摘要：A simple and accurate method for simultaneously quantifying two co-occurring Fusarium mycotoxins in rice and bran, nivalenol (NIV) and deoxynivalenol (DON), is described. The method involves the use of an immunoaffinity column for cleanup and HPLC-UV detection for quantification. The limits of quantification were −1 for the two toxins in rice and bran. The mean recoveries from blank samples spiked at levels of 100–1000 μg kg−1 were 86.2–106.6% for NIV and 93.1–106.2% for DON, with relative standard deviations of 6–15% for NIV and 3–11% for DON, respectively. The detection rate of NIV in 482 rice and 239 bran samples was 34–96%, where the level ranging from 5.7 to 2791.4 μg kg−1, whereas that for DON was 10.4–44.8% with levels ranging from 7.1 to 655.6 μg kg−1. The co-occurrence rates of NIV and DON were 9.1%, 14.9%, and 41.5% for white rice, brown rice, and bran, respectively. The estimated dietary intakes of NIV and DON for the Korean population based on the occurrence data were well below the established tolerable daily intake. 
外文关键词：Bran；Brown rice；Dietary intake；Deoxynivalenol；Nivalenol；Simultaneous determination；White rice
作者：Ok, HE；Lee, SY；Chun, HS
作者单位：Chung Ang Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2018
出版刊次：5
点击下载：通过HPLC-UV检测及免疫亲和纯化研究精米和米糠中雪腐镰刀菌烯醇及脱氧雪腐镰刀菌烯醇的发生并进行同步测定