中文摘要：本研究旨在研发基于适体的FRET检测策略，用来特异性灵敏检测污染粮食中的AFB1。结果表明，所研发的方法在常规食品检测实验室对AFB1的风险评估有很好的实用价值。
外文摘要：The present study aimed to develop an aptamer-based FRET detection strategy for the specific and sensitive detection of AFB1 in contaminated food grains. The study comprises generation of ssDNA aptamers against AFB1 by whole-cell SELEX and their application in a FRET-based platform utilizing graphene oxide (GO) and quantum dots (QDs). The generated aptamers were characterized to determine their specificity and sensitivity using indirect ELISA where AFB1–OVA was used as a coating antigen. Among the aptamers generated, the ATB1 aptamer showed good reactivity and selectivity against AFB1. This aptamer was further characterized to determine its secondary structure and KD value, which was found to be 5.9 kcal mol−1. The characterized aptamers were conjugated onto Cd/Se quantum dots to develop a fluorimetric system for the detection of aflatoxin B1 using a graphene oxide platform. The presence of graphene oxide quenches the fluorescence ability of the quantum dots due to π–π stacking interactions between the aptamer and GO. Upon target addition, the aptamer forms a complex with aflatoxin B1 thereby restoring the fluorescence intensity. The developed assay shows a linear response from 0.002 μg μl−1 to 0.2 μg μl−1 with a detection limit of 0.004 μg μl−1 for the AFB1 standard toxin and showed no cross-reactivity with other closely related mycotoxins. To validate the reliability of the developed method, several field samples spiked with AFB1 were included in this study and the results obtained were cross verified using a standard commercial AFB1 kit. In conclusion, the developed method may find good utility in routine food testing laboratories for risk assessment of AFB1.
作者：Kumar, YVVA；Renuka, RM；Achuth, J；Mudili, V；Poda, S
作者单位：Acharya Nagarjuna Univ
期刊名称：RSC ADVANCES
期刊影响因子：3.289
出版年份：2018
出版刊次：19
点击下载：研发FRET荧光传感器，检测污染粮食样品中的黄曲霉毒素B1