中文摘要：黄曲霉毒素（AFs）是剧毒化合物，能引起人类急性和慢性中性。黄曲霉毒素B1（AFB1）是毒性最强的AFs。因此，AFB1的现场快速检测是食品安全管理的关键。本研究报道了运用便携式表面等离子体共振（SPR）传感器现场检测谷物中的AFB1。研究发现，通过运用这种便携式SPR竞争性免疫检测，传感器的检测限(2.51 ppb)及定量限(16.32 ppb)均很低。此外，我们还成功地检测了水稻、花生及杏仁样品中的AFB1，表明该传感方法可用于食品中霉菌毒素的现场监测。
外文摘要：Aflatoxins (AFs) are highly toxic compounds that can cause both acute and chronic toxicity in humans. Aflatoxin B1 (AFB1) is considered the most toxic of AFs. Therefore, the rapid and on-site detection of AFB1 is critical for food safety management. Here, we report the on-site detection of AFB1 in grains by a portable surface plasmon resonance (SPR) sensor. For the detection of AFB1, the surface of an SPR Au chip was sequentially modified by cysteine-protein G, AFB1 antibody, and bovine serum albumin (BSA). Then, the sample solution and AFB1-BSA conjugate were flowed onto the Au chip in serial order. In the absence of AFB1, the SPR response greatly increased due to the binding of AFB1-BSA on the Au chip. In the presence of AFB1, the SPR response showed little change because the small AFB1 molecule binds on the Au chip instead of the large AFB1-BSA molecule. By using this portable SPR-based competitive immunoassay, the sensor showed low limits of detection (2.51 ppb) and quantification (16.32 ppb). Furthermore, we successfully detected AFB1 in rice, peanut, and almond samples, which suggests that the proposed sensing method can potentially be applied to the on-site monitoring of mycotoxins in food. 
外文关键词：aflatoxin B1；portable SPR；competitive immunoassay；sensor；on-site detection
作者：Moon, J；Byun, J；Kim, H；Lim, EK；Jeong, J；Jung, J；Kang, T
作者单位：KRIBB
期刊名称：SENSORS
期刊影响因子：2.033
出版年份：2018
出版刊次：2
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