中文摘要：玉米赤霉烯酮（ZEN）是谷物中常见的一种雌激素样霉菌毒素。为了满足ZEN超灵敏检测的需要，本研究以G蛋白（PG）为传感界面的识别粘合剂，研发了两种新型纳米磁珠(MNBs)荧光免疫分析法。并用市售ELISA试剂盒评估所研制的方法的性能，结果令人满意。
外文摘要：Zearalenone (ZEN) is a type of estrogenic mycotoxin commonly found in cereals. In order to satisfy the need for ultrasensitive detection of ZEN, we developed two novel magnetic nanobeads (MNBs)-based fluoroimmunoassays using protein G (PG) as recognition binder on the sensing interface. One proposed facile strategy is based on a first capture last react (FCLR) procedure while the other is a first react last capture (FRLC) format. Specifically, CdTe/CdS/ZnS quantum dots were synthesized and modified to antigen (OVA-ZEN) as the signal probes. The PG modified MNBs specifically captured the fragment crystallizable region of immunoglobulin G (IgG) with a level of orientation while avoiding the destruction of antibody's binding sites caused by chemical coupling. Under the optimized conditions, the detection limits of 0.019 ng mL(-1) and 0.049 ng mL(-1) in the extract solution were obtained for the FCLR and FRLC, respectively. Furthermore, the established methods proved to be successful in detecting ZEN in real cereal samples with the detection limits being 0.6 mu g kg(-1) and 1.5 mu g kg(-1) in the FCLR and FRLC, respectively. The performance of the proposed assays was evaluated utilizing commercial ELISA kits with satisfactory results.
外文关键词：Zearalenone；Fluoroimmunoassay；Quantum dots；Magnetic nanobeads；Protein G
作者：Zhang, FY；Liu, B；Liu, GZ；Sheng, W；Zhang, Y；Liu, Q；Wang, S
作者单位：Tianjin Univ Sci & Technol
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2018
出版刊次：10
点击下载：用以G蛋白为识别标记的新型纳米磁珠荧光免疫分析法检测谷物中的玉米赤霉烯酮