中文摘要：本研究运用一种竞争型光电化学(PEC)免疫传感器成功地测定了黄曲霉毒素B1（AFB1）。研究表明，该生物传感器具有良好的重现性，可接受的稳定性及高特异性，表明其在其他毒性小分子检测中具有潜在的应用前景。
外文摘要：A competitive photoelectrochemical (PEC) immunosensor was successfully utilized for quantitative detection of aflatoxin B1 (AFB1). The flower-like tungsten oxide (WO3) hierarchical architectures with large specific surface areas and porous nanostructure was prepared as a matrix material for immobilizing of BSA-AFB1. The Ag ions-modified bismuth vanadate nano-polyhedron (BiVO4@Ag+) was utilized as labels for immobilization anti-AFB1 through affinity specific binding. The Ag2S was prepared in-situ growth by immediately deposition the S2− onto the BiVO4@Ag+. The high photocurrent intensity was generated by the obtained BiVO4@Ag2S under visible-light irradiation. Moreover, excellent sensitivity was achieved by using the WO3 as the matrix and BiVO4@Ag2S as labels to enhance photocurrent response and improve the photocurrent conversion efficiency. Under optimal conditions, the competitive PEC immunosensor showed wide linear range from 1.0 pg mL−1 to 120 ng mL−1 with limit detection of 0.28 pg mL−1 for AFB1. The biosensor was performed with good reproducibility, acceptable stability, and high specificity, which indicated potential application in the detection of other toxic small molecules.
外文关键词：Competitive type photoelectrochemical immunosensor；BiVO4 nano polyhedrons；Flower-like WO3；Aflatoxin B1
作者：Feng, JH；Li, YY；Gao, ZQ；Lv, H；Zhang, XB；Dong, YH；Wang, P；Fan, DW； Wei, Q
作者单位：Shandong Univ Technol
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2018
出版刊次：10
点击下载：用一种以花状WO3为基质，Ag2S增强BIOV4进行信号放大的竞争型光电化学免疫传感器检测黄曲霉毒素B1