中文摘要：本文以F-couSiPc作三（2,2'-联吡啶－N，N′）、二氯，六水合物（II）（Ru（BPY）（3）（2 +））的共反应促进剂，TiO2 MOFs为载体材料构建了一种有效的集成电致化学发光(ECL)生物探针，用来研制一种竞争型ECL方法，检测脱氧雪腐镰刀菌烯醇（DON）。本研究基于竞争性免疫反应，构建的简便灵敏的ECL免疫传感器，检测DON的线性范围从0.1pg/mL到20ng/mL，检测限为0.03pg/mL (S/N= 3)，性能优良。
外文摘要：Herein, an effective integrated electrochemiluminescence (ECL) bioprobe was constructed based on F-couSiPc as co-reaction accelerator of tris (2,2'-bipyridine-n,n')-, dichloride, hexahydrate (II) (Ru(bpy)(3)(2+)) and TiO2 MOFs as carrier for developing a competitive-type ECL strategy for the sensing of deoxynivalenol (DON). Initially, TiO2 MOFs with high specific surface area and regular pore structure was employed to immobilize Ru(bpy)(3)(2+) and Mercapto-beta-cyclodextrin (beta-CD), then sensitizer of F-couSiPc was introduced into the system by encapsulating into the cup-shaped cavity of beta-CD, which mainly ascribed to the supermolecular self-assembly behavior, perfectly avoiding the aggregation of F-couSiPc. Due to the shortened electronic transmission distance, reduced energy loss and less reagent consumption, the integrated ECL reaction pattern between the luminescent Ru(bpy)(3)(2+) and sensitizer of F-couSiPc has shown improved luminous efficiency compared with the common intermolecular ECL reactions. Meanwhile, the (3-Aminopropyl) triethoxysilane (APTES), perylene-3,4,9,10-tetracarboxylic acid (PTCA) and carbon nanohorn (CNHs) nanohybrid (APTES-PTCA@CNHs), combing the remarkable advantages of CNHs with the desirable electronic and optical properties of PTCA, served as matrix to immobilize antigen (DON) with an increasing amount, and on account of the high affinity between the APTES and DON, a stable ECL performance was obtained. Based on the competitive immunoreactions, a facile and sensitive ECL immunosensor was constructed for the detection of DON, with excellent linear range from 0.1 pg/mL to 20 ng/mL and a detection limit of 0.03 pg/mL (S/N= 3).
外文关键词：Competitive ECL immunosensor；Integrated ECL bioprobe；TiO2 MOFs；F-couSiPc；Deoxynivalenol (DON)
作者：Zheng, HL；Yi, H；Dai, H；Fang, DD；Hong, ZS；Lin, DM；Zheng, XQ；Lin, YY
作者单位：Fujian Normal Univ
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2018
出版刊次：10
点击下载：基于TiO2 MOFs的氟香豆素硅酞菁敏化集成电致化学发光生物探针用于检测脱氧雪腐镰刀菌烯醇