中文摘要：本研究研制了一种新型高灵敏磁性酶化学发光免疫分析法(MECLIA)，用来检测谷物样品中的伏马菌素B1（FB1）。研究表明，该MECLIA法为高通量筛查谷物中的伏马菌素提供了一种潜在的应用。
外文摘要：BACKGROUND In this work, a novel highly sensitive magnetic enzyme chemiluminescence immunoassay (MECLIA) was developed to detect fumonisin B1 (FB1) in cereal samples. The gold-coated magnetic nanoparticles (Fe3O4@Au, GoldMag) were used as solid phase carrier to develop a competitive CLIA for detecting FB1, in which FB1 in samples would compete with FB1-OVA coated on the surface of Fe3O4@Au nanoparticles in binding with FB1 antibodies. Successively, horseradish peroxidase labeled goat anti-rabbit IgG (HRP-IgG) was conjugated with FB1 antibodies on the microplate. In substrate solution containing luminol and H2O2, HRP-IgG catalyzed luminol oxidation by H2O2, generating a high chemiluminescence signal. The FB1 immune GoldMag particles were characterized by Fourier transform infrared (IR) spectroscopy, scanning electron microscope, zeta potential analysis, etc. RESULTS The concentrations and the reaction times of these immunoreagents were optimized to improve the performances of this method. The established method could detect as low as 0.027 ng·mL−1 FB1 from 0.05 ng·mL−1 to 25 ng·mL−1 with little cross-reaction (less than 2.4%) to other structurally related compounds. The average intrassay RSD (n = 6) was 3.4% and average interassay RSD (n = 6) was 5.4%. This method was successfully applied to the determination of FB1 in corn and wheat and gave recoveries between 98%-110% and 91%-105%, respectively. CONCLUSION Our results suggested that this MECLIA approach provided a potential application for high throughput fumonisin screening in cereals.
外文关键词：magnetic enzyme chemiluminescence immunoassay, fumonisin B-1, Fe3O4@Au nanoparticles, cereal analysis
作者：Jie, MS；Yu, SC；Yu, F；Liu, L；He, LL；Li, YQ；Zhang, HQ；Qu, LB； Harrington, PD；Wu, YJ
作者单位：Zhengzhou Univ
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2018
出版刊次：9
点击下载：用基于金包被磁性纳米颗粒的超灵敏化学发光免疫分析检测谷物中的伏马菌素B1