中文摘要：作者描述了一种光电化学(PEC)免疫分析法，用来测定食品中的黄曲霉毒素B1（AFB1）。用商业AFB1 ELISA试剂盒作对照，通过分析自然污染及加标的花生样品，评估了该方法的准确性，得到的结果与对照一致。
外文摘要：The authors describe a photoelectrochemical (PEC) immunoassay for determination of aflatoxin B1 (AFB1) in foodstuff. The competitive immunoreaction is carried out on a microplate coated with a capture antibody against AFB1 using AFB1-bovine serum albumin (BSA)-liposome-coated mesoporous silica nanoparticles (MSN) loaded with L-cysteine as a support. The photocurrent is produced by a photoactive material consisting of cerium-doped Bi2MoO6. Initially, L-cysteine acting as the electron donor is gated in the pores by interaction between mesoporous silica and liposome. Thereafter, AFB1-BSA conjugates are covalently bound to the liposomes. Upon introduction of the analyte (AFB1), the labeled AFB1-BSA complex competes with the analyte for the antibody deposited on the microplate. Accompanying with the immunocomplex, the liposomes on the MSNs are lysed upon addition of Triton X-100. This results in the opening of the pores and in a release of L-cysteine. Free cysteine then induces the electron-hole scavenger of the photoactive nanosheets to increase the photocurrent. The photocurrent (relative to background signal) increases with increasing AFB1 concentration. Under optimum conditions, the photoactive nanosheets display good photoelectrochemical responses, and allow the detection of AFB1 at a concentration as low as 0.1 pg·mL-1 within a linear response in the 0.3 pg·mL-1 to 10 ng·mL-1 concentration range. Accuracy was evaluated by analyzing naturally contaminated and spiked peanut samples by using a commercial AFB1 ELISA kit as the reference, and well-matching results were obtained.
外文关键词：Photoelectrochemical immunoassay；Aflatoxin B-1；Ce-doped Bi2MoO6 nanosheets；Peanut sample
作者：Lin, YX；Zhou, Q；Zeng, YY；Tang, DP
作者单位：Fuzhou Univ
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2018
出版刊次：6
点击下载：脂质体包覆介孔二氧化硅纳米颗粒负载L-半胱氨酸用于黄曲霉毒素B1的光电免疫测定